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Slc12a5 / KCC2 Recombinant Mouse Monoclonal Antibody [PSH13-20] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610271

Slc12a5 / KCC2 Recombinant Mouse Monoclonal Antibody [PSH13-20] - BSA and Azide free

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Slc12a5 / KCC2 Recombinant Mouse Monoclonal Antibody [PSH13-20] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Rat Slc12a5 / KCC2 aa 932-1043.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 126 kDa

Positive Control

Mouse hippocampus tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH13-20

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • WB

  • 1:5,000

Target

Function

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl- homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl- levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation.

Background References

1. Tomita K et al. Therapeutic potential for KCC2-targeted neurological diseases. Jpn Dent Sci Rev. 2023 Dec

2. Hu JJ et al. Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus. Nat Neurosci. 2023 May

Subcellular Location

Cell membrane, Cell projection, dendrite.

UNIPROT

SwissProt: Q91V14 Mouse

SwissProt: Q63633 Rat

Synonyms

Electroneutral potassium chloride cotransporter 2 antibody

Electroneutral potassium-chloride cotransporter 2 antibody

Erythroid K Cl cotransporter 2 antibody

Furosemide sensitive K Cl cotransporter antibody

hKCC2 antibody

K-Cl cotransporter 2 antibody

KCC 2 antibody

KCC2 antibody

KIAA1176 antibody

Neuronal K Cl cotransporter antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Western blot analysis of Slc12a5 / KCC2 on different lysates with Mouse anti-Slc12a5 / KCC2 antibody (<a href="/products/HA610271" style="font-weight: bold;text-decoration: underline;">HA610271</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse hippocampus tissue lysate (no heat)<br />Lane 2: Mouse brain tissue lysate (no heat)<br />Lane 3: Rat brain tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 126 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610271" style="font-weight: bold;text-decoration: underline;">HA610271</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Slc12a5 / KCC2 on different lysates with Mouse anti-Slc12a5 / KCC2 antibody (HA610271) at 1/5,000 dilution.

    Lane 1: Mouse hippocampus tissue lysate (no heat)
    Lane 2: Mouse brain tissue lysate (no heat)
    Lane 3: Rat brain tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 126 kDa
    Observed band size: 150 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610271) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"