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Kv4.2 / KCND2 Recombinant Mouse Monoclonal Antibody [PSH13-21] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610272

Kv4.2 / KCND2 Recombinant Mouse Monoclonal Antibody [PSH13-21] - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Kv4.2 / KCND2 Recombinant Mouse Monoclonal Antibody [PSH13-21] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Rat Kv4.2 / KCND2 aa 207-226.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Molecular Weight

Predicted band size: 70 kDa

Positive Control

Mouse cerebellum tissue, rat cerebellum tissue, Mouse cerebellum tissue lysate, Mouse hippocampus tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate.

Conjugation

unconjugated

Clone Number

PSH13-21

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

  • WB

  • 1:5,000

Target

Function

Potassium voltage-gated channel subfamily D member 2 is a protein that in humans is encoded by the KCND2 gene. It contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential. Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member mediates a rapidly inactivating, A-type outward potassium current which is not under the control of the N terminus as it is in Shaker channels.

Background References

1. Zhou H et al. KCND2: A prognostic biomarker and regulator of immune function in gastric cancer. Cancer Med. 2023 Aug

2. Zhang Y et al. KCND2 variants associated with global developmental delay differentially impair Kv4.2 channel gating. Hum Mol Genet. 2021 Nov

Subcellular Location

Cell membrane, Cell projection, dendrite, Synapse, Perikaryon, Postsynaptic cell membrane, Cell projection, Cell junction.

UNIPROT

SwissProt: Q9Z0V2 Mouse

SwissProt: Q63881 Rat

Synonyms

KCD2 antibody

KCND 2 antibody

KCND2 antibody

KCND2_HUMAN antibody

KIAA1044 antibody

MGC119702 antibody

MGC119703 antibody

Potassium voltage gated channel Shal related subfamily member 2 antibody

Potassium voltage-gated channel subfamily D member 2 antibody

RK 5 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (<a href="/products/HA610272" style="font-weight: bold;text-decoration: underline;">HA610272</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610272" style="font-weight: bold;text-decoration: underline;">HA610272</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA610272) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610272) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (<a href="/products/HA610272" style="font-weight: bold;text-decoration: underline;">HA610272</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610272" style="font-weight: bold;text-decoration: underline;">HA610272</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA610272) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610272) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Kv4.2 / KCND2 on different lysates with Mouse anti-Kv4.2 / KCND2 antibody (<a href="/products/HA610272" style="font-weight: bold;text-decoration: underline;">HA610272</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse cerebellum tissue lysate (no heat)<br />Lane 2: Mouse hippocampus tissue lysate (no heat)<br />Lane 3: Mouse brain tissue lysate (no heat)<br />Lane 4: Mouse liver tissue lysate (negative)<br />Lane 5: Rat cerebellum tissue lysate<br />Lane 6: Rat liver tissue lysate (negative)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 71 kDa<br />Observed band size: 71 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610272" style="font-weight: bold;text-decoration: underline;">HA610272</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Kv4.2 / KCND2 on different lysates with Mouse anti-Kv4.2 / KCND2 antibody (HA610272) at 1/5,000 dilution.

    Lane 1: Mouse cerebellum tissue lysate (no heat)
    Lane 2: Mouse hippocampus tissue lysate (no heat)
    Lane 3: Mouse brain tissue lysate (no heat)
    Lane 4: Mouse liver tissue lysate (negative)
    Lane 5: Rat cerebellum tissue lysate
    Lane 6: Rat liver tissue lysate (negative)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 71 kDa
    Observed band size: 71 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610272) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"