Kv4.2 / KCND2 Recombinant Mouse Monoclonal Antibody [PSH13-21]
Catalog# HA601434
Kv4.2 / KCND2 Recombinant Mouse Monoclonal Antibody [PSH13-21]
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IHC-Fr
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IHC-P
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WB
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Mouse
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Rat
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unconjugated
Overview
Product Name
Kv4.2 / KCND2 Recombinant Mouse Monoclonal Antibody [PSH13-21]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Rat Kv4.2 / KCND2 aa 207-226.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, IHC-P, WB
Target Molecular Weight
Predicted band size: 70 kDa
Positive Control
Mouse cerebellum tissue, rat cerebellum tissue, Mouse cerebellum tissue lysate, Mouse hippocampus tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate.
Conjugation
unconjugated
Clone Number
PSH13-21
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:500
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IHC-P
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1:1,000
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WB
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1:5,000
Target
Function
Potassium voltage-gated channel subfamily D member 2 is a protein that in humans is encoded by the KCND2 gene. It contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential. Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member mediates a rapidly inactivating, A-type outward potassium current which is not under the control of the N terminus as it is in Shaker channels.
Background References
1. Zhou H et al. KCND2: A prognostic biomarker and regulator of immune function in gastric cancer. Cancer Med. 2023 Aug
2. Zhang Y et al. KCND2 variants associated with global developmental delay differentially impair Kv4.2 channel gating. Hum Mol Genet. 2021 Nov
Subcellular Location
Cell membrane, Cell projection, dendrite, Synapse, Perikaryon, Postsynaptic cell membrane, Cell projection, Cell junction.
Synonyms
KCD2 antibody
KCND 2 antibody
KCND2 antibody
KCND2_HUMAN antibody
KIAA1044 antibody
MGC119702 antibody
MGC119703 antibody
Potassium voltage gated channel Shal related subfamily member 2 antibody
Potassium voltage-gated channel subfamily D member 2 antibody
RK 5 antibody
ExpandImages
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Application: IHC-Fr
Species: Rat
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA601434) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601434) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA601434) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601434) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of Kv4.2 / KCND2 on different lysates with Mouse anti-Kv4.2 / KCND2 antibody (HA601434) at 1/5,000 dilution.
Lane 1: Mouse cerebellum tissue lysate (no heat)
Lane 2: Mouse hippocampus tissue lysate (no heat)
Lane 3: Mouse brain tissue lysate (no heat)
Lane 4: Mouse liver tissue lysate (negative)
Lane 5: Rat cerebellum tissue lysate
Lane 6: Rat liver tissue lysate (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601434) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"