ROR1 Recombinant Mouse Monoclonal Antibody [PSH13-99] - BSA and Azide free
Catalog# HA610281
ROR1 Recombinant Mouse Monoclonal Antibody [PSH13-99] - BSA and Azide free
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WB
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FC
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Human
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HA601447
含抗保成分
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unconjugated
Overview
Product Name
ROR1 Recombinant Mouse Monoclonal Antibody [PSH13-99] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human ROR1 aa 428-937.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 104 kDa
Positive Control
PANC-1 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, NCCIT cell lysate, K-562 cell lysate, K-562 (left, negative) and NCCIT (right, positive).
Conjugation
unconjugated
Clone Number
PSH13-99
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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FC
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1:1,000
Target
Function
Tyrosine-protein kinase transmembrane receptor ROR1, also known as neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), is an enzyme that in humans is encoded by the ROR1 gene. ROR1 is a member of the receptor tyrosine kinase-like orphan receptor (ROR) family. The protein encoded by this gene is a receptor tyrosine kinase that modulates neurite growth in the central nervous system. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. ROR1 is currently under investigation for its role in the metastasis of cancer cells.
Background References
1. Osorio-Rodríguez DA et al. Anti-ROR1 CAR-T cells: Architecture and performance. Front Med (Lausanne). 2023 Feb
2. Quezada MJ et al. The signaling pathways activated by ROR1 in cancer. Cell Signal. 2023 Apr
Subcellular Location
Membrane, Cell projection, axon.
UNIPROT
Synonyms
dJ537F10.1 antibody
Inactive tyrosine protein kinase transmembrane receptor ROR1 antibody
MGC99659 antibody
Neurotrophic tyrosine kinase antibody
Neurotrophic tyrosine kinase receptor related 1 antibody
Neurotrophic tyrosine kinase, receptor related 1 antibody
NTRKR1 antibody
OTTHUMP00000010573 antibody
OTTHUMP00000010574 antibody
OTTMUSP00000008344 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of ROR1 on different lysates with Rabbit anti-ROR1 antibody (HA610281) at 1/5,000 dilution.
Lane 1: PANC-1 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (negative) (20 µg/Lane)
Lane 3: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 4: NCCIT cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (negative) (20 µg/Lane)
Predicted band size: 104 kDa
Observed band size: 130 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610281) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Flow cytometric analysis of K-562 (left, negative) and NCCIT (right, positive) cells labeling ROR1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA610281, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"