Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Catalog# HA610286
Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
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IHC-Fr
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WB
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Mouse
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Rat
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HA601452
含抗保成分
-
unconjugated
Overview
Product Name
Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within mouse Mannose Receptor(CD206) aa 1-1,409.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, WB
Molecular Weight
Predicted band size: 165 kDa
Positive Control
Mouse lung tissue lysate, Mouse liver tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
PSH07-76
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:500
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WB
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1:5,000
Target
Function
The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). The receptor recognises terminal mannose, N-acetylglucosamine and fucose residues on glycans attached to proteins found on the surface of some microorganisms, playing a role in both the innate and adaptive immune systems. Additional functions include clearance of glycoproteins from circulation, including sulphated glycoprotein hormones and glycoproteins released in response to pathological events. The mannose receptor recycles continuously between the plasma membrane and endosomal compartments in a clathrin-dependent manner.
Background References
1. Nielsen MC et al. Macrophage Activation Markers, CD163 and CD206, in Acute-on-Chronic Liver Failure. Cells. 2020 May
2. Nawaz A et al. Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration. Nat Commun. 2022 Nov
Subcellular Location
Endosome membrane, Cell membrane.
Synonyms
bA541I19.1 antibody
C type lectin domain family 13 member D antibody
C-type lectin domain family 13 member D antibody
CD 206 antibody
CD206 antibody
CD206 antigen antibody
CLEC13D antibody
CLEC13DL antibody
Macrophage mannose receptor 1 antibody
Macrophage mannose receptor 1 like protein 1 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: liver
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: liver
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Western blot analysis of Mannose Receptor(CD206) on different lysates with Guinea pig anti-Mannose Receptor(CD206) antibody (HA610286) at 1/2,000 dilution.
Lane 1: Mouse lung tissue lysate (20 µg/Lane)
Lane 2: Mouse liver tissue lysate (20 µg/Lane)
Lane 4: Rat lung tissue lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 200 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610286) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"