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Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric)

Usd: 350

Specification

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Catalog# HA601452

Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric)

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Mannose Receptor(CD206) Recombinant Antibody [PSH07-76] - Guinea pig IgG2 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within mouse Mannose Receptor(CD206) aa 1-1,409.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 165 kDa

Positive Control

Mouse lung tissue lysate, Mouse liver tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH07-76

Reactivity Data

IHC-Fr WB
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • WB

  • 1:5,000

Target

Function

The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). The receptor recognises terminal mannose, N-acetylglucosamine and fucose residues on glycans attached to proteins found on the surface of some microorganisms, playing a role in both the innate and adaptive immune systems. Additional functions include clearance of glycoproteins from circulation, including sulphated glycoprotein hormones and glycoproteins released in response to pathological events. The mannose receptor recycles continuously between the plasma membrane and endosomal compartments in a clathrin-dependent manner.

Background References

1. Nielsen MC et al. Macrophage Activation Markers, CD163 and CD206, in Acute-on-Chronic Liver Failure. Cells. 2020 May

2. Nawaz A et al. Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration. Nat Commun. 2022 Nov

Subcellular Location

Endosome membrane, Cell membrane.

UNIPROT

SwissProt: Q61830 Mouse

Entrez Gene: 291327 Rat

Synonyms

bA541I19.1 antibody

C type lectin domain family 13 member D antibody

C-type lectin domain family 13 member D antibody

CD 206 antibody

CD206 antibody

CD206 antigen antibody

CLEC13D antibody

CLEC13DL antibody

Macrophage mannose receptor 1 antibody

Macrophage mannose receptor 1 like protein 1 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: liver<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: liver

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: liver<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: liver

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Western blot analysis of Mannose Receptor(CD206) on different lysates with Guinea pig anti-Mannose Receptor(CD206) antibody (<a href="/products/HA601452" style="font-weight: bold;text-decoration: underline;">HA601452</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate (20 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (20 µg/Lane)<br />Lane 4: Rat lung tissue lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 165 kDa<br />Observed band size: 200 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601452" style="font-weight: bold;text-decoration: underline;">HA601452</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Mannose Receptor(CD206) on different lysates with Guinea pig anti-Mannose Receptor(CD206) antibody (HA601452) at 1/2,000 dilution.

    Lane 1: Mouse lung tissue lysate (20 µg/Lane)
    Lane 2: Mouse liver tissue lysate (20 µg/Lane)
    Lane 4: Rat lung tissue lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (20 µg/Lane)

    Predicted band size: 165 kDa
    Observed band size: 200 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601452) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation