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VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Mouse IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610311

VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Mouse IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within N terminal Mouse VAChT.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P

Target Molecular Weight

Predicted band size: 57 kDa

Positive Control

Mouse brain (striatum) tissue, rat brain (striatum) tissue.

Conjugation

unconjugated

Clone Number

PSH10-50

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The Vesicular acetylcholine transporter (VAChT) is a neurotransmitter transporter which is responsible for loading acetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene, located within the first intron of the choline acetyltransferase gene. VAChT is able to transport ACh into vesicles by relying on an exchange between protons (H+) that were previously pumped into the vesicle diffusing out, thus acting as an antiporter. ACh molecules are then carried into the vesicle by the action of exiting protons. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase.

Background References

1. Zhang Y et al. Structural insights into VAChT neurotransmitter recognition and inhibition. Cell Res. 2024 Sep

2. Pergolizzi S et al. Expression of VAChT and 5-HT in Ulcerative colitis dendritic cells. Acta Histochem. 2021 May

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.

UNIPROT

SwissProt: O35304 Mouse

SwissProt: Q62666 Rat

Synonyms

AChR antibody

MGC12716 antibody

rVAT antibody

Slc18a3 antibody

Solute carrier family 18 (vesicular acetylcholine) member 3 antibody

Solute carrier family 18 (vesicular monoamine) member 3 antibody

Solute carrier family 18 member 3 antibody

VAChT antibody

VACHT_HUMAN antibody

Vesicular acetylcholine transporter antibody

Images

  • This data was developed using <a href="/products/HA601477" style="font-weight: bold;text-decoration: underline;">HA601477</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain (striatum)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    This data was developed using HA601477, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: brain (striatum)

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601477" style="font-weight: bold;text-decoration: underline;">HA601477</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (<a href="/products/HA601477" style="font-weight: bold;text-decoration: underline;">HA601477</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601477" style="font-weight: bold;text-decoration: underline;">HA601477</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601477, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (HA601477) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601477) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601477" style="font-weight: bold;text-decoration: underline;">HA601477</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (<a href="/products/HA601477" style="font-weight: bold;text-decoration: underline;">HA601477</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601477" style="font-weight: bold;text-decoration: underline;">HA601477</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601477, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (HA601477) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601477) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"