VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Mouse IgG1 (Chimeric)
Catalog# HA601477
VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Mouse IgG1 (Chimeric)
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IHC-Fr
-
IHC-P
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Mouse
-
Rat
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unconjugated
Overview
Product Name
VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within N terminal Mouse VAChT.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, IHC-P
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
Mouse brain (striatum) tissue, rat brain (striatum) tissue.
Conjugation
unconjugated
Clone Number
PSH10-50
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| IHC-P | IHC-Fr | |
|---|---|---|
| mouse |
|
|
| rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-Fr
-
1:200
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IHC-P
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1:500
Target
Function
The Vesicular acetylcholine transporter (VAChT) is a neurotransmitter transporter which is responsible for loading acetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene, located within the first intron of the choline acetyltransferase gene. VAChT is able to transport ACh into vesicles by relying on an exchange between protons (H+) that were previously pumped into the vesicle diffusing out, thus acting as an antiporter. ACh molecules are then carried into the vesicle by the action of exiting protons. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase.
Background References
1. Zhang Y et al. Structural insights into VAChT neurotransmitter recognition and inhibition. Cell Res. 2024 Sep
2. Pergolizzi S et al. Expression of VAChT and 5-HT in Ulcerative colitis dendritic cells. Acta Histochem. 2021 May
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
Synonyms
AChR antibody
MGC12716 antibody
rVAT antibody
Slc18a3 antibody
Solute carrier family 18 (vesicular acetylcholine) member 3 antibody
Solute carrier family 18 (vesicular monoamine) member 3 antibody
Solute carrier family 18 member 3 antibody
VAChT antibody
VACHT_HUMAN antibody
Vesicular acetylcholine transporter antibody
Images
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Application: IHC-Fr
Species: Mouse
Site: brain (striatum)
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (HA601477) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601477) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (HA601477) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601477) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"