EAAT2 Recombinant Antibody [PS01-62] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Catalog# HA610334
EAAT2 Recombinant Antibody [PS01-62] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
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IHC-Fr
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WB
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Mouse
-
Rat
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HA601504
含抗保成分
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unconjugated
Overview
Product Name
EAAT2 Recombinant Antibody [PS01-62] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human EAAT2
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, WB
Molecular Weight
Predicted band size: 62 kDa
Positive Control
Mouse brain tissue lysate.
Conjugation
unconjugated
Clone Number
PS01-62
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:200
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WB
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1:5,000
Target
Function
Excitatory amino acid transporter 2 (EAAT2) also known as solute carrier family 1 member 2 (SLC1A2) and glutamate transporter 1 (GLT-1) is a protein that in humans is encoded by the SLC1A2 gene. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. SLC1A2 / EAAT2 is a member of a family of the solute carrier family of proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. EAAT2 is responsible for over 90% of glutamate reuptake within the brain.
Background References
1. Blacker CJ et al. EAAT2 as a Research Target in Bipolar Disorder and Unipolar Depression: A Systematic Review. Mol Neuropsychiatry. 2020 Apr
2. Green JL et al. Role of glutamate excitotoxicity and glutamate transporter EAAT2 in epilepsy: Opportunities for novel therapeutics development. Biochem Pharmacol. 2021 Nov
Subcellular Location
Cell membrane.
Synonyms
EAA2_HUMAN antibody
EAAT2 antibody
Excitatory amino acid transporter 2 antibody
Excitotoxic amino acid transporter 2 antibody
Glial high affinity glutamate transporter antibody
GLT 1 antibody
GLT1 antibody
Glutamate aspartate transporter II antibody
Glutamate transporter 1 antibody
Glutamate/aspartate transporter II antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
☑ Relative expression (RE)
Western blot analysis of EAAT2 on different lysates with Guinea pig anti-EAAT2 antibody (HA610334) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse lung tissue lysate (negative) (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610334) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"