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SATB2 Recombinant Antibody [JM52-44] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610338

SATB2 Recombinant Antibody [JM52-44] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SATB2 Recombinant Antibody [JM52-44] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human SATB2 238-287 / 733.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, WB

Target Molecular Weight

Predicted band size: 83 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JM52-44

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

SATB2 (Special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.

Background References

1. Gu W et al. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Cell Stem Cell. 2022 Jan

2. Roy SK et al. SATB2 is a novel biomarker and therapeutic target for cancer. J Cell Mol Med. 2020 Oct

Subcellular Location

Nucleus matrix.

UNIPROT

SwissProt: Q9UPW6 Human

SwissProt: Q8VI24 Mouse

Entrez Gene: 501145 Rat

Synonyms

DNA binding protein SATB2 antibody

DNA-binding protein SATB2 antibody

FLJ21474 antibody

FLJ32076 antibody

GLSS antibody

KIAA1034 antibody

MGC119474 antibody

MGC119477 antibody

SATB family member 2 antibody

SATB homeobox 2 antibody

Expand

Images

  • This data was developed using <a href="/products/HA601508" style="font-weight: bold;text-decoration: underline;">HA601508</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    This data was developed using HA601508, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601508" style="font-weight: bold;text-decoration: underline;">HA601508</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    This data was developed using HA601508, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601508" style="font-weight: bold;text-decoration: underline;">HA601508</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of SATB2 on different lysates with Guinea pig anti-SATB2 antibody (<a href="/products/HA601508" style="font-weight: bold;text-decoration: underline;">HA601508</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (10 µg/Lane)<br />Lane 2: HeLa cell lysate (10 µg/Lane)<br />Lane 3: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (negative) (20 µg/Lane)<br />Lane 5: Rat brain tissue lysate (20 µg/Lane)<br />Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 83 kDa<br />Observed band size: 83 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601508" style="font-weight: bold;text-decoration: underline;">HA601508</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA601508, the same antibody clone in a different buffer formulation.
    Western blot analysis of SATB2 on different lysates with Guinea pig anti-SATB2 antibody (HA601508) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate (10 µg/Lane)
    Lane 2: HeLa cell lysate (10 µg/Lane)
    Lane 3: Mouse brain tissue lysate (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (negative) (20 µg/Lane)
    Lane 5: Rat brain tissue lysate (20 µg/Lane)
    Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601508) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"