SATB2 Recombinant Antibody [JM52-44] - Guinea pig IgG2 (Chimeric)
Catalog# HA601508
SATB2 Recombinant Antibody [JM52-44] - Guinea pig IgG2 (Chimeric)
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IHC-Fr
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SATB2 Recombinant Antibody [JM52-44] - Guinea pig IgG2 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human SATB2 238-287 / 733.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-Fr, WB
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JM52-44
Reactivity Data
| IHC-Fr | WB | |
|---|---|---|
| Human |
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| Mouse |
|
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| Rat |
|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:500
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WB
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1:5,000
Target
Function
SATB2 (Special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.
Background References
1. Gu W et al. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Cell Stem Cell. 2022 Jan
2. Roy SK et al. SATB2 is a novel biomarker and therapeutic target for cancer. J Cell Mol Med. 2020 Oct
Subcellular Location
Nucleus matrix.
Synonyms
DNA binding protein SATB2 antibody
DNA-binding protein SATB2 antibody
FLJ21474 antibody
FLJ32076 antibody
GLSS antibody
KIAA1034 antibody
MGC119474 antibody
MGC119477 antibody
SATB family member 2 antibody
SATB homeobox 2 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Western blot analysis of SATB2 on different lysates with Guinea pig anti-SATB2 antibody (HA601508) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (negative) (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601508) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"