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Aldolase A/B/C Recombinant Antibody [PSH01-81] - Rat IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610342

Aldolase A/B/C Recombinant Antibody [PSH01-81] - Rat IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Aldolase A/B/C Recombinant Antibody [PSH01-81] - Rat IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human Aldolase C aa 50-100.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Target Molecular Weight

Predicted band size: 40 kDa

Positive Control

Mouse brain tissue, rat brain tissue, U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH01-81

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:2,000

  • WB

  • 1:5,000

Target

Function

ALDOC is a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, Aldolase C is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively.

Background References

1. Chen L, Zeng Y, Ren B, et al. ALDOC regulated the biological function and immune infiltration of gastric cancer cells. Int J Biochem Cell Biol. 2023 May; 158:106407.

2. Fan K, Wang J, Sun W, et al. MUC16 C-terminal binding with ALDOC disrupts the ability of ALDOC to sense glucose and promotes gallbladder carcinoma growth. Exp Cell Res. 2020 Sep 1;394(1):112118.

Subcellular Location

cytoskeleton, cytosol, extracellular exosome, extracellular region

UNIPROT

SwissProt: P09972 Human

SwissProt: P05063 Mouse

SwissProt: P09117 Rat

Synonyms

ALDC antibody

ALDO C antibody

aldoc antibody

ALDOC_HUMAN antibody

Aldolase 3 antibody

Aldolase C Fructose bisphosphate antibody

Brain type aldolase antibody

Brain-type aldolase antibody

Fructoaldolase C antibody

Fructose 1 6 biphosphate triosephosphate lyase antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Aldolase A/B/C antibody (<a href="/products/HA610342" style="font-weight: bold;text-decoration: underline;">HA610342</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610342" style="font-weight: bold;text-decoration: underline;">HA610342</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Aldolase A/B/C antibody (HA610342) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610342) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Aldolase A/B/C antibody (<a href="/products/HA610342" style="font-weight: bold;text-decoration: underline;">HA610342</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610342" style="font-weight: bold;text-decoration: underline;">HA610342</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Aldolase A/B/C antibody (HA610342) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610342) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Aldolase A/B/C on different lysates with Rat anti-Aldolase A/B/C antibody (<a href="/products/HA610342" style="font-weight: bold;text-decoration: underline;">HA610342</a>) at 1/5,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: RAW264.7 cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: Mouse brain tissue lysate<br />Lane 5: Mouse pancreas tissue lysate (negative)<br />Lane 6: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610342" style="font-weight: bold;text-decoration: underline;">HA610342</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Aldolase A/B/C on different lysates with Rat anti-Aldolase A/B/C antibody (HA610342) at 1/5,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: RAW264.7 cell lysate
    Lane 3: C6 cell lysate
    Lane 4: Mouse brain tissue lysate
    Lane 5: Mouse pancreas tissue lysate (negative)
    Lane 6: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610342) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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