PCSK9 Mouse Monoclonal Antibody [2F1] - BSA and Azide free
Catalog# HA610371
PCSK9 Mouse Monoclonal Antibody [2F1] - BSA and Azide free
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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EM1701-26
含抗保成分
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unconjugated
Overview
Product Name
PCSK9 Mouse Monoclonal Antibody [2F1] - BSA and Azide free
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein with Human PCSK9 1-201 / 692.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Cell, FC
Molecular Weight
Predicted band size: 74 kDa
Positive Control
PANC-1, H22, SW1990, HepG2, human liver tissue, human colon cancer tissue, human kidney tissue, mouse kidney tissue.
Conjugation
unconjugated
Clone Number
2F1
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG2b
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IF-Cell
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1:200-1:500
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IHC-P
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1:200-1:500
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FC
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1:500-1:1,000
Target
Function
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC-1, is a 692 amino acid protein that belongs to the peptidase S8 family and contains one peptidase S8 domain. Important in the regulation of plasma cholesterol homeostasis, PCSK9 binds to low-density lipid receptor family members LDLR, very low-density lipid receptor (VLDLR) and apolipoprotein receptor 2 (ApoER2) and promotes their degradation in intracellular acidic compartments. PCSK9 also plays a role in neuronal differentiation and apoptosis. PCSK9 is expressed in Shwann cells, neuro-epithelioma, colon carcinoma, and hepatic and pancreatic cell lines. PCSK9 levels in the brain are highest in the cerebellum during perinatal development, with ischemia causing increased levels in the adult brain. Defects in the gene encoding this protein causes the autosomal dominant disorder familial hypercholesterolemia 3 (HCHOLA3).
Background References
1. Lai Q et al. E2F1 inhibits circulating cholesterol clearance by regulating Pcsk9 expression in the liver. JCI Insight 2:10 (2017).
2. Wen S et al. Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice. J Lipids 2013:908048 (2013).
Sequence Similarity
Belongs to the peptidase S8 family.
Tissue Specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Post-translational Modification
Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation.; Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein.; Phosphorylation protects the propeptide against proteolysis.
Subcellular Location
Secreted, endoplasmic reticulum,lysosome, cytoplasm.
Synonyms
Convertase subtilisin/kexin type 9 preproprotein antibody
FH3 antibody
HCHOLA3 antibody
Hypercholesterolemia autosomal dominant 3 antibody
LDLCQ1 antibody
NARC 1 antibody
NARC-1 antibody
NARC1 antibody
Neural apoptosis regulated convertase 1 antibody
Neural apoptosis-regulated convertase 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of PCSK9 on different lysates with Mouse anti-PCSK9 antibody (HA610371) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: K-562 cell lysate
Lane 3: SH-SY5Y cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 62,78 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling PCSK9 with Mouse anti-PCSK9 antibody (HA610371) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCSK9 antibody (HA610371) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon caner tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of HepG2 cells labeling PCSK9.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA610371, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"