PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50] - BSA and Azide free
Catalog# HA610397
PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
Mouse heart tissue lysate, Mouse liver tissue lysate, Rat placenta tissue lysate, Rat heart tissue lysate, HeLa treated with 10μM CCCP for 24 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH22-50
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
A member of the serine / threonine protein kinase family, PTEN induced putative kinase 1 (PINK1) is a tumor suppressor. PINK1 is primarily located in mitochondria, and is ubiquitously expressed in testis, skeletal muscle, and heart tissue. at lower levels in pancreas, ovary, brain, placenta, kidney, liver, prostate and small intestine. During cellular stress PINK1 protects against mitochondrial dysfunction by inducing phosphorylation mitochondrial proteins. PINK1 mutations may give rise to different autophosphorylation activity. Mutations in the PINK1 gene (PARK6) are associated with early onset Parkinson's disease, a recessive neurodegenerative disorder characterized by resting tremor, muscular rigidity, bradykinesia and postural instability. Parkinson's disease generally involves the presence of intraneuronal accumulations of aggregated proteins (Lewy bodies) in brain neurons.
Background References
1. Quinn PMJ et al. PINK1/PARKIN signalling in neurodegeneration and neuroinflammation. Acta Neuropathol Commun. 2020 Nov
2. Han R et al. PINK1-PRKN mediated mitophagy: differences between in vitro and in vivo models. Autophagy. 2023 May
Subcellular Location
SwissProt: Q9BXM7 Human SwissProt: Q99MQ3 Mouse SwissProt: B5DFG1 Rat
Synonyms
BRPK antibody
FLJ27236 antibody
mitochondrial antibody
PARK 6 antibody
PARK6 antibody
Phosphatase and Tensin Homolog antibody
PINK 1 antibody
PINK1 antibody
PINK1_HUMAN antibody
Protein kinase BRPK antibody
ExpandImages
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This data was developed using HA601588, the same antibody clone in a different buffer formulation.
Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA601588) at 1/5,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat placenta tissue lysate
Lane 4: Rat heart tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA601588, the same antibody clone in a different buffer formulation.
Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA601588) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]
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