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PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]

Usd: 385

Specification

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Catalog# HA601588

PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 63 kDa

Positive Control

Mouse heart tissue lysate, Mouse liver tissue lysate, Rat placenta tissue lysate, Rat heart tissue lysate, HeLa treated with 10μM CCCP for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH22-50

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

A member of the serine / threonine protein kinase family, PTEN induced putative kinase 1 (PINK1) is a tumor suppressor. PINK1 is primarily located in mitochondria, and is ubiquitously expressed in testis, skeletal muscle, and heart tissue. at lower levels in pancreas, ovary, brain, placenta, kidney, liver, prostate and small intestine. During cellular stress PINK1 protects against mitochondrial dysfunction by inducing phosphorylation mitochondrial proteins. PINK1 mutations may give rise to different autophosphorylation activity. Mutations in the PINK1 gene (PARK6) are associated with early onset Parkinson's disease, a recessive neurodegenerative disorder characterized by resting tremor, muscular rigidity, bradykinesia and postural instability. Parkinson's disease generally involves the presence of intraneuronal accumulations of aggregated proteins (Lewy bodies) in brain neurons.

Background References

1. Quinn PMJ et al. PINK1/PARKIN signalling in neurodegeneration and neuroinflammation. Acta Neuropathol Commun. 2020 Nov

2. Han R et al. PINK1-PRKN mediated mitophagy: differences between in vitro and in vivo models. Autophagy. 2023 May

Subcellular Location

SwissProt: Q9BXM7 Human SwissProt: Q99MQ3 Mouse SwissProt: B5DFG1 Rat

UNIPROT

SwissProt: Q9BXM7 Human

SwissProt: Q99MQ3 Mouse

SwissProt: B5DFG1 Rat

Synonyms

BRPK antibody

FLJ27236 antibody

mitochondrial antibody

PARK 6 antibody

PARK6 antibody

Phosphatase and Tensin Homolog antibody

PINK 1 antibody

PINK1 antibody

PINK1_HUMAN antibody

Protein kinase BRPK antibody

Expand

Images

  • Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (<a href="/products/HA601588" style="font-weight: bold;text-decoration: underline;">HA601588</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse heart tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Rat placenta tissue lysate<br />Lane 4: Rat heart tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601588" style="font-weight: bold;text-decoration: underline;">HA601588</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA601588) at 1/5,000 dilution.

    Lane 1: Mouse heart tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Rat placenta tissue lysate
    Lane 4: Rat heart tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (<a href="/products/HA601588" style="font-weight: bold;text-decoration: underline;">HA601588</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601588" style="font-weight: bold;text-decoration: underline;">HA601588</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA601588) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601588) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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