DUSP6 Recombinant Antibody [SR39-09] - Mouse IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610456
DUSP6 Recombinant Antibody [SR39-09] - Mouse IgG1 (Chimeric) - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
DUSP6 Recombinant Antibody [SR39-09] - Mouse IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Human DUSP6 aa 325-381 / 381.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
Hep G2 (Human hepatocellular carcinoma cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
SR39-09
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Dual specificity phosphatase 6 (DUSP6) is an enzyme that in humans is encoded by the DUSP6 gene. The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas and, unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. Upregulation of MKP-3 has been shown to alleviate chronic postoperative pain.
Background References
1. Bertin S et al. Dual-specificity phosphatase 6 regulates CD4(+) T-cell functions and restrains spontaneous colitis in IL-10-deficient mice. Mucosal Immunol N/A:N/A (2014).
2. Wales S et al. Global MEF2 target gene analysis in cardiac and skeletal muscle reveals novel regulation of DUSP6 by p38MAPK-MEF2 signaling. Nucleic Acids Res 42:11349-62 (2014).
Subcellular Location
Cytoplasm.
Synonyms
Dual specificity phosphatase 6 antibody
Dual specificity phosphatase 6 isoform a antibody
Dual specificity protein phosphatase 6 antibody
Dual specificity protein phosphatase PYST1 antibody
DUS6_HUMAN antibody
DUSP 6 antibody
DUSP 6a antibody
Dusp6 antibody
DUSP6a antibody
HH19 antibody
ExpandImages
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This data was developed using HA601675, the same antibody clone in a different buffer formulation.
Western blot analysis of DUSP6 on different lysates with Mouse anti-DUSP6 antibody (HA601675) at 1/5,000 dilution.
Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601675, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 42 kDa
Observed band size: 42 kDa -
This data was developed using HA601675, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Pancreas
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601675, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
This data was developed using HA601675, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Pancreas
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601675, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
DUSP6 Recombinant Antibody [SR39-09] - Mouse IgG1 (Chimeric)
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
DUSP6 Recombinant Rabbit Monoclonal Antibody [SR39-09]
Application: WB,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat,Chicken
Conjugate: unconjugated
