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HRP Conjugated DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) Alpaca Monoclonal Antibody

Usd: 300

Specification

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Catalog# HA710083

HRP Conjugated DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) Alpaca Monoclonal Antibody

Application

  • WB

  • ELISA

Reactivity
Conjugation

  • HRP

Overview

Product Name

HRP Conjugated DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) Alpaca Monoclonal Antibody

Antibody Type

Alpaca Nanobody

Immunogen

DYKDDDDK tag fusion protein

Product Specificity

DYKDDDDK tag sequence

Validated Applications

WB, ELISA

Conjugation

HRP

Product Features

Form

Liquid

Concentration

1mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Store at –20 ℃, (Avoid freeze / thaw cycles), protect from light

Storage Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Isotype

Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation)

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • ELISA

  • 1:5000 -1:20000

  • WB

  • 1:5000 -1:20000

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) is based on recombinant, mouse IgG2b Fc fused fab of alpaca IgG1 to DYKDDDDK tag coupled to HRP. Based on western blot and ELISA, Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) reacts with the DYKDDDDK tag selectively, no reactivity with other proteins., High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .

Background References

暂无

Images

  • Western blot analysis of DYKDDDDK tag on different lysates with Mouse anti-DYKDDDDK tag antibody (<a href="/products/HA710083" style="font-weight: bold;text-decoration: underline;">HA710083</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate<br />Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA710083" style="font-weight: bold;text-decoration: underline;">HA710083</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of DYKDDDDK tag on different lysates with Mouse anti-DYKDDDDK tag antibody (HA710083) at 1/5,000 dilution.

    Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate
    Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710083) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  • Advantages of using an Alpaca Recombinant Secondary<br />1. Smaller size<br />Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.<br />2. Easier to use<br />Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. <br />3. Easier production<br />When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. <br />4. More consistent<br />Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.<br />5. Higher Affinity<br />Nanobodies benefit from consistently high binding affinity values.

    Advantages of using an Alpaca Recombinant Secondary
    1. Smaller size
    Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
    2. Easier to use
    Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
    3. Easier production
    When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
    4. More consistent
    Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
    5. Higher Affinity
    Nanobodies benefit from consistently high binding affinity values.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"