HRP Conjugated Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody(HRP) Antibody (HA710083)
Catalog# HA710083
Alpaca Monoclonal to DYKDDDDK tag
Tested for
-
WB
-
ELISA
Conjugated to HRP
Western blot analysis of DYKDDDDK tag on different lysates with Mouse anti-DYKDDDDK tag antibody (HA710083) at 1/5,000 dilution.
Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate
Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710083) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate
Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710083) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
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