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DYKDDDDK tag, AlpHcAbs® Mouse antibody Alpaca Monoclonal

Usd: 300

Specification

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Catalog# HA710084

DYKDDDDK tag, AlpHcAbs® Mouse antibody Alpaca Monoclonal

Application

  • WB

  • ICC/IF

  • ELISA

  • IP

Reactivity
Conjugation

  • unconjugated

Overview

Product Name

DYKDDDDK tag, AlpHcAbs® Mouse antibody Alpaca Monoclonal

Antibody Type

Alpaca Nanobody

Immunogen

DYKDDDDK tag fusion protein

Product Specificity

DYKDDDDK tag sequence

Validated Applications

WB, ICC/IF, ELISA, IP

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

1mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Store at –20 ℃, (Avoid freeze / thaw cycles), Stable for 12 months at -20℃

Storage Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Isotype

Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation)

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • ELISA

  • 1:5,000 -1:20,000

  • WB

  • 1:5,000 -1:20,000

  • ICC/IF

  • 1:200-1:1,000

  • IP

  • 2ug/sample

  • Flow Cyt

  • 1:200-1:1,000

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody is recombinant fab of alpaca IgG1 fused to Mouse IgG2b Fc. Based on western blot and ELISA, Anti-DYKDDDDK tag, AlpHcAbs® Mouse antibody reacts with the DYKDDDDK tag selectively, no reactivity with other proteins. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .

Background References

暂无

Images

  • Western blot analysis of DYKDDDDK tag on different lysates with Mouse anti-DYKDDDDK tag antibody (<a href="/products/HA710084" style="font-weight: bold;text-decoration: underline;">HA710084</a>) at 1/10,000 dilution.<br /><br />Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate<br />Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA710084" style="font-weight: bold;text-decoration: underline;">HA710084</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DYKDDDDK tag on different lysates with Mouse anti-DYKDDDDK tag antibody (HA710084) at 1/10,000 dilution.

    Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate
    Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710084) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot-Anti-DYKDDDDK tag, AlpHcAbs&reg; Mouse IgG2b antibody(<a href="/products/HA710084" style="font-weight: bold;text-decoration: underline;">HA710084</a>)<br />Anti-DYKDDDDK tag, AlpHcAbs&reg; Mouse IgG2b antibody(<a href="/products/HA710084" style="font-weight: bold;text-decoration: underline;">HA710084</a>) at 0.1 µg/ml<br />Lane1:<br />N-terminal DYKDDDDK fusion protein <br />Lane2:<br />C-terminal DYKDDDDK fusion protein<br /><br />Secondary antibody：<br />Anti-Mouse IgG-HRP(<a href="/products/HA710142" style="font-weight: bold;text-decoration: underline;">HA710142</a>)(1:10000)

    Western blot-Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(HA710084)
    Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(HA710084) at 0.1 µg/ml
    Lane1:
    N-terminal DYKDDDDK fusion protein
    Lane2:
    C-terminal DYKDDDDK fusion protein

    Secondary antibody：
    Anti-Mouse IgG-HRP(HA710142)(1:10000)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"