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HRP Conjugated His tag, AlpHcAbs® Rabbit antibody(HRP) Alpaca Monoclonal Antibody

Usd: 300

Specification

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Catalog# HA710110

HRP Conjugated His tag, AlpHcAbs® Rabbit antibody(HRP) Alpaca Monoclonal Antibody

Application

  • WB

  • ELISA

Reactivity
Conjugation

  • HRP

Overview

Product Name

HRP Conjugated His tag, AlpHcAbs® Rabbit antibody(HRP) Alpaca Monoclonal Antibody

Antibody Type

Alpaca Nanobody

Immunogen

6*His tag fusion protein

Product Specificity

His tag sequence(HHHHHH)

Validated Applications

WB, ELISA

Conjugation

HRP

Product Features

Form

Liquid

Concentration

1mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Store at –20 ℃, (Avoid freeze / thaw cycles), protect from light,

Storage Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Isotype

Fab of alpaca IgG1 fused to Rabbit Fc(mutation)

Purification Method

Recombinant expression and Affinity purified

Application Dilution

  • ELISA

  • 1:5000 -1:20000

  • WB

  • 1:5000 -1:20000

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-His tag, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 to His tag coupled to HRP, and Anti-His tag, AlpHcAbs® Rabbit antibody(HRP) detects the His tag selectively, no reactivity with other proteins. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .

Background References

暂无

Images

  • Western blot analysis of His tag on different lysates with Rabbit anti-His tag antibody (<a href="/products/HA710110" style="font-weight: bold;text-decoration: underline;">HA710110</a>) at 1/20,000 dilution.<br /><br />Lane 1: 293T transfected with His-tagged Claudin 18.2 (N-terminal) cell lysate (no heat)<br />Lane 2: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 5 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA710110" style="font-weight: bold;text-decoration: underline;">HA710110</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of His tag on different lysates with Rabbit anti-His tag antibody (HA710110) at 1/20,000 dilution.

    Lane 1: 293T transfected with His-tagged Claudin 18.2 (N-terminal) cell lysate (no heat)
    Lane 2: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 5 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710110) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  • Advantages of using an Alpaca Recombinant Secondary<br />1. Smaller size<br />Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.<br />2. Easier to use<br />Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use. <br />3. Easier production<br />When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies. <br />4. More consistent<br />Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.<br />5. Higher Affinity<br />Nanobodies benefit from consistently high binding affinity values.

    Advantages of using an Alpaca Recombinant Secondary
    1. Smaller size
    Nanobodies are 1/10 the size of a traditional antibody (~15 kDa unconjugated). This means that the nanobody can penetrate tissue faster and deeper than other antibodies.
    2. Easier to use
    Due to their simple structure, nanobodies also have the advantage of increased stability and ease of use.
    3. Easier production
    When compared to a more traditional antibody, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
    4. More consistent
    Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift.
    5. Higher Affinity
    Nanobodies benefit from consistently high binding affinity values.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"