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HA tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal

Usd: 300

Specification

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Catalog# HA710116

HA tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal

Application

  • WB

  • ICC/IF

  • ELISA

  • IP

Reactivity
Conjugation

  • unconjugated

Overview

Product Name

HA tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal

Antibody Type

Alpaca Nanobody

Immunogen

HA tag fused KLH

Product Specificity

HA tag(YPYDVPDYA)

Validated Applications

WB, ICC/IF, ELISA, IP

Conjugation

unconjugated

Product Features

Concentration

Storage Instructions

Store at –20 ℃(Avoid freeze / thaw cycles)

Storage Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Isotype

VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • WB

  • 1:5,000-1:20000

  • ELISA

  • 1:5,000-1:20000

  • IP

  • 1-2ug/sample,

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-HA tag, AlpHcAbs® Rabbit antibody is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and Anti-HA tag, AlpHcAbs® Rabbit antibody detects the HA tag selectively, no reactivity with other proteins., High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .

Background References

暂无

Images

  • Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (<a href="/products/HA710116" style="font-weight: bold;text-decoration: underline;">HA710116</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate<br />Lane 2: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate<br />Lane 3: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate<br />Lane 4: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA710116" style="font-weight: bold;text-decoration: underline;">HA710116</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (HA710116) at 1/5,000 dilution.

    Lane 1: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
    Lane 2: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate
    Lane 3: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate
    Lane 4: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710116) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot-Anti-HA tag, AlpHcAbs&reg; Mouse antibody(<a href="/products/HA710116" style="font-weight: bold;text-decoration: underline;">HA710116</a>)<br />Anti-HA tag, AlpHcAbs&reg; Mouse antibody(<a href="/products/HA710116" style="font-weight: bold;text-decoration: underline;">HA710116</a>) at 0.1 µg/ml<br />Lane1:<br />N-terminal HA tag fusion protein<br />Lane2:<br />C-terminal HA tag fusion protein<br /><br />Secondary antibody：<br />Anti-Mouse IgG-HRP(<a href="/products/HA710142" style="font-weight: bold;text-decoration: underline;">HA710142</a>)(1:10000)

    Western blot-Anti-HA tag, AlpHcAbs® Mouse antibody(HA710116)
    Anti-HA tag, AlpHcAbs® Mouse antibody(HA710116) at 0.1 µg/ml
    Lane1:
    N-terminal HA tag fusion protein
    Lane2:
    C-terminal HA tag fusion protein

    Secondary antibody：
    Anti-Mouse IgG-HRP(HA710142)(1:10000)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"