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Myc tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal

Usd: 300

Specification

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Catalog# HA710120

Myc tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal

Application

  • WB

  • ICC/IF

  • ELISA

  • IP

Reactivity
Conjugation

  • unconjugated

Overview

Product Name

Myc tag, AlpHcAbs® Rabbit antibody Alpaca Monoclonal

Antibody Type

Alpaca Nanobody

Immunogen

Myc tag fusion protein

Product Specificity

Myc tag sequence(EQKLISEEDL)

Validated Applications

WB, ICC/IF, ELISA, IP

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at –20 ℃, (Avoid freeze / thaw cycles), Stable for 12 months at -20℃

Storage Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Isotype

Fab of alpaca IgG1 fused to Rabbit Fc(mutation)

Purification Method

Recombinant Expression and Affinity purified

Application Dilution

  • WB

  • 1:10,000-1:50,000

  • ELISA

  • 1:10,000-1:50,000

  • ICC/IF

  • 1:200-1:1000IP: 2ug/sample

  • Flow Cyt

  • 1:200-1:2000,

  • Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Target

Function

Anti-Myc tag, AlpHcAbs® Rabbit antibody is designed for detecting Myc tag fusion protein specifically. Anti-Myc tag, AlpHcAbs® Rabbit antibody is recombinant fab of alpaca IgG1 fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-Myc tag, AlpHcAbs® Rabbit antibody reacts with the Myc tag sequence(EQKLISEEDL) selectively, no reactivity with other proteins.   High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .

Background References

暂无

Images

  • Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>) at 1/10,000 dilution.<br /><br />Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)<br />Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs&reg; VHH(HRP) Secondary Antibody (<a href="/products/HA710051" style="font-weight: bold;text-decoration: underline;">HA710051</a>) at 1/10,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA710120) at 1/10,000 dilution.

    Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)
    Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710120) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710051) at 1/10,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>) at 1/10,000 dilution.<br /><br />Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)<br />Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs&reg; VHH(HRP) Secondary Antibody (<a href="/products/HA710049" style="font-weight: bold;text-decoration: underline;">HA710049</a>) at 1/10,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA710120) at 1/10,000 dilution.

    Lane 1: 293T transfected with Myc-tagged Claudin18.2 cell lysate (no heat)
    Lane 2: 293T transfected with Myc-tagged Histone H3.1 cell lysate

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710120) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710049) at 1/10,000 dilution was used for 1 hour at room temperature.

  • Western blot-Anti-Myc tag, AlpHcAbs&reg; Rabbit antibody(<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>)<br />Anti-Myc tag, AlpHcAbs&reg; Rabbit antibody(<a href="/products/HA710120" style="font-weight: bold;text-decoration: underline;">HA710120</a>) at 0.1 µg/ml<br />Lane1:<br />N-terminal Myc tag fusion protein<br />Lane2:<br />C-terminal Myc tag fusion protein<br /><br />Secondary antibody：<br />Anti-Rabbit IgG-HRP(<a href="/products/HA710046" style="font-weight: bold;text-decoration: underline;">HA710046</a>)(1:10000)

    Western blot-Anti-Myc tag, AlpHcAbs® Rabbit antibody(HA710120)
    Anti-Myc tag, AlpHcAbs® Rabbit antibody(HA710120) at 0.1 µg/ml
    Lane1:
    N-terminal Myc tag fusion protein
    Lane2:
    C-terminal Myc tag fusion protein

    Secondary antibody：
    Anti-Rabbit IgG-HRP(HA710046)(1:10000)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"