Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody
Catalog# HA710156
Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody
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WB
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ELISA
Overview
Product Name
Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) Alpaca Monoclonal Antibody
Antibody Type
Alpaca Nanobody
Immunogen
Recombinant mouse IgG
Product Specificity
Mouse IgG(Fcγ fragment specific)
Validated Applications
WB, ELISA
Product Features
Concentration
1mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Store at –20 ℃(Avoid freeze / thaw cycles), protect from light, Stable for 12 months at -20℃
Storage Buffer
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Isotype
VHH domain of alpaca IgG2b/2c
Purification Method
Recombinant Expression and Affinity purified
Application Dilution
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ELISA
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1:10,000-1:50,000
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WB
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1:10,000-1:50,000
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Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Target
Function
Anti-Mouse IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is designed for detecting the Fc region of mouse IgG. Anti-Mouse IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is based on monovalent, recombinant single domain antibodies to mouse IgG(Fcγ fragment specific) coupled to HRP, and the Anti-Mouse IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) detects the Fc region of mouse IgG selectively, no reactivity with mouse IgM, or the Fab portion of mouse immunoglobulins., ·High lot-to-lot consistency·Increased sensitivity and higher affinity·Animal-free production . . . .
Background References
暂无
Images
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ELISA of specificity for different species of IgG-Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody. -
A titer ELISA of mouse IgG-Anti-Mouse IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP)
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG or different isotope of mouse IgG. 1:10000 dilution of Anti-Mouse IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody. -
Western blot analysis of Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP) on C-FLAG protein with Mouse anti-FLAG antibody (HA601167) at 1/2,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 1 minute 7 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601167) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Mouse IgG(H+L), AlpSdAbs® VHH(HRP) Secondary Antibody (HA710156) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"