HRP Conjugated Mouse IgG for IP, AlpSdAbs® VHH(HRP) Mouse IgG Monoclonal Antibody
Catalog# HA710160
HRP Conjugated Mouse IgG for IP, AlpSdAbs® VHH(HRP) Mouse IgG Monoclonal Antibody
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WB
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HRP
Overview
Product Name
HRP Conjugated Mouse IgG for IP, AlpSdAbs® VHH(HRP) Mouse IgG Monoclonal Antibody
Antibody Type
Alpaca Nanobody
Immunogen
Mouse IgG
Product Specificity
Non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3)
Validated Applications
WB
Conjugation
HRP
Product Features
Concentration
1mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Storage Buffer
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Isotype
VHH domain of alpaca IgG2b/2c
Purification Method
Recombinant Expression and Affinity purified
Application Dilution
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WB
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1:5,000-1:10,000,
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Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Target
Function
Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) is designed for detecting the non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3). Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) is based on Monovalent, recombinant single domain antibody to mouse IgG coupled to HRP, and the Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, using Anti-mouse IgG for IP, AlpSdAbs™ VHH(HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains. ·High lot-to-lot consistency·Increased sensitivity and higher affinity·Animal-free production . . . .
Background References
暂无
Images
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Western blot-Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP)(HA710160)
Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP)(HA710160) at 0.1 µg/ml
Lane 1 : Mouse IgG1(non-reduced)
Lane 2 : Mouse IgG1(reduced)
Lane 3 : Mouse IgG2a(non-reduced)
Lane 4 : Mouse IgG2a(reduced)
Lane 5 : Mouse IgG2b(non-reduced)
Lane 6 : Mouse IgG2b(reduced)
Lane 7 : Mouse IgG3(non-reduced)
Lane 8 : Mouse IgG3(reduced)
Mouse IgG at 0.2 µg per lane.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"