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IGF2BP2 Recombinant Rabbit Monoclonal Antibody [JE56-58]

Usd: 385

Specification

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Catalog# HA720003

IGF2BP2 Recombinant Rabbit Monoclonal Antibody [JE56-58]

Application

  • WB

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IGF2BP2 Recombinant Rabbit Monoclonal Antibody [JE56-58]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human IGF2BP2 aa 100-200.

Species Reactivity

Human, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 66 kDa

Positive Control

293T cell lysate, Jurkat cell lysate, rat bone marrow tissue lysates.

Conjugation

unconjugated

Clone Number

JE56-58

RRID

AB_3071944

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

Target

Function

This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes.

Background References

1. Hu X. et. al. IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader. Cell Death Differ. 2020 Jun

2. Wang Y. et. al. LncRNA LINRIS stabilizes IGF2BP2 and promotes the aerobic glycolysis in colorectal cancer. Mol Cancer. 2019 Dec

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9Y6M1 Human

Synonyms

Hepatocellular carcinoma autoantigen p62 antibody

IF2B2_HUMAN antibody

IGF 2 mRNA binding protein 2 antibody

IGF II mRNA binding protein 2 antibody

IGF-II mRNA-binding protein 2 antibody

IGF2 mRNA-binding protein 2 antibody

Igf2bp2 antibody

IMP 2 antibody

IMP-2 antibody

IMP2 antibody

Expand

Images

  • Western blot analysis of IGF2BP2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA720003" style="font-weight: bold;text-decoration: underline;">HA720003</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: 293T cell lysate<br />Lane 2: Jurkat cell lysate

    Western blot analysis of IGF2BP2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 293T cell lysate
    Lane 2: Jurkat cell lysate

  • Western blot analysis of IGF2BP2 on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA720003" style="font-weight: bold;text-decoration: underline;">HA720003</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IGF2BP2 on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation