ACAA2 Recombinant Rabbit Monoclonal Antibody [JE56-67]
Catalog# HA720006
ACAA2 Recombinant Rabbit Monoclonal Antibody [JE56-67]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ACAA2 Recombinant Rabbit Monoclonal Antibody [JE56-67]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human ACAA2 aa 347-397.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 42 kDa
Positive Control
293T cell lysate, rat colon tissue lysate, NIH/3T3 cell lysate, rat kidney tissue, human liver tissue, human appendix tissue.
Conjugation
unconjugated
Clone Number
JE56-67
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
Target
Function
ACAA2 Antibody (192) is a high quality monoclonal ACAA2 antibody (also designated ACAA2 antibody) suitable for the detection of the ACAA2 protein of mouse, rat and human origin. ACAA2 Antibody (192) is available as the non-conjugated anti-ACAA2 antibody. Mammalian tissues contain five types of thiolases, all of which participate in metabolism of various compounds throughout the body. ACAA2 (acetyl-Coenzyme A acyltransferase 2), also known as DSAEC, is a 397 amino acid member of the thiolase family of enzymes and is involved in lipid metabolism. Localized to the mitochondrion, ACAA2 catalyzes the last step, namely the conversion of acetyl-CoA to 3-oxoacyl-CoA, in the fatty acid oxidation pathway. ACAA2 is highly expressed in liver, fibrofblasts and intercostal muscle and contains an N-terminal targeting signal that, unlike other mitochondrial proteins, is non-cleavable. Human ACAA2 shares 86.6% amino acid identity with its rat counterpart, suggesting a conserved function for ACAA2 among different species.
Background References
1. Symeou S. et. al. ACAA2 and FASN polymorphisms affect the fatty acid profile of Chios sheep milk. J Dairy Res. 2020 Feb
2. Yang Y. et. al. MiR-152 Regulates Apoptosis and Triglyceride Production in MECs via Targeting ACAA2 and HSD17B12 Genes. Sci Rep. 2018 Jan
Subcellular Location
Mitochondrion.
Synonyms
3 ketoacyl CoA thiolase antibody
3 ketoacyl CoA thiolase mitochondrial antibody
3-ketoacyl-CoA thiolase, mitochondrial antibody
3-oxoacyl-CoA thiolase, mitochondrial antibody
ACAA 2 antibody
Acaa2 antibody
Acetyl CoA acyltransferase 2 antibody
Acetyl CoA acyltransferase antibody
Acetyl Coenzyme A acyltransferase 2 antibody
Acetyl-CoA acyltransferase antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of ACAA2 on different lysates with Rabbit anti-ACAA2 antibody (HA720006) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si ACAA2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720006) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ACAA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720006, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate
Lane 2: Rat colon tissue lysate
Lane 3: NIH/3T3 cell lysate -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-ACAA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720006, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"