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NCS1 Recombinant Rabbit Monoclonal Antibody [JE56-39]

Usd: 385

Specification

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Catalog# HA720017

NCS1 Recombinant Rabbit Monoclonal Antibody [JE56-39]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

NCS1 Recombinant Rabbit Monoclonal Antibody [JE56-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NCS1 aa 90-190.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 22 kDa

Positive Control

SH-SY5Y cell lysate, A549 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE56-39

RRID

AB_3071957

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Angebault C. et. al. ER-mitochondria cross-talk is regulated by the Ca(2+) sensor NCS1 and is impaired in Wolfram syndrome. Sci Signal. 2018 Oct

2. Nguyen LD. et. al. Characterization of NCS1-InsP3R1 interaction and its functional significance. J Biol Chem. 2019 Dec

Subcellular Location

Cell membrane, Golgi apparatus, postsynaptic density, perinuclear region, Cytoplasm, Membrane.

UNIPROT

SwissProt: P62166 Human

SwissProt: Q8BNY6 Mouse

SwissProt: P62168 Rat

Synonyms

9430075O15Rik antibody

A730032G13Rik antibody

AI836659 antibody

DKFZp761L1223 antibody

FLUP antibody

FREQ antibody

Frequenin antibody

Frequenin homolog (Drosophila) antibody

Frequenin homolog antibody

Frequenin like protein antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of NCS1 on different lysates with Rabbit anti-NCS1 antibody (<a href="/products/HA720017" style="font-weight: bold;text-decoration: underline;">HA720017</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-NCS1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720017" style="font-weight: bold;text-decoration: underline;">HA720017</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of NCS1 on different lysates with Rabbit anti-NCS1 antibody (HA720017) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-NCS1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720017) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of NCS1 on different lysates with Rabbit anti-NCS1 antibody (<a href="/products/HA720017" style="font-weight: bold;text-decoration: underline;">HA720017</a>) at 1/5,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: Neuro-2a cell lysate<br />Lane 4: Mouse brain tissue lysate<br />Lane 5: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720017" style="font-weight: bold;text-decoration: underline;">HA720017</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NCS1 on different lysates with Rabbit anti-NCS1 antibody (HA720017) at 1/5,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: A549 cell lysate
    Lane 3: Neuro-2a cell lysate
    Lane 4: Mouse brain tissue lysate
    Lane 5: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720017) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"