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TUB Recombinant Rabbit Monoclonal Antibody [JE58-75]

Usd: 385

Specification

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Catalog# HA720035

TUB Recombinant Rabbit Monoclonal Antibody [JE58-75]

Application

  • WB

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TUB Recombinant Rabbit Monoclonal Antibody [JE58-75]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human TUB aa 1-50/506.

Species Reactivity

Human, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 56 kDa

Positive Control

HepG2 cell lysate, L6 cell lysate.

Conjugation

unconjugated

Clone Number

JE58-75

RRID

AB_3071975

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.

Background References

1. Wang RX. et. al. A serine protease homologue Bombyx mori scarface induces a short and fat body shape in silkworm. Insect Mol Biol. 2018 Jun

Subcellular Location

Cytoplasm, Nucleus, Secreted, Cell membrane.

UNIPROT

SwissProt: P50607 Human

SwissProt: P50586 Mouse

SwissProt: O88808 Rat

Synonyms

F10B5.4 antibody

rd5 antibody

TUB 1 antibody

TUB antibody

TUB_HUMAN antibody

Tubby homologue antibody

Tubby protein homolog 1 antibody

Tubby protein homolog antibody

Images

  • Western blot analysis of TUB on different lysates with Rabbit anti-TUB antibody (<a href="/products/HA720035" style="font-weight: bold;text-decoration: underline;">HA720035</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: L6 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: Lane 1: 42 seconds; Lane 2: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720035" style="font-weight: bold;text-decoration: underline;">HA720035</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TUB on different lysates with Rabbit anti-TUB antibody (HA720035) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: L6 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 65 kDa

    Exposure time: Lane 1: 42 seconds; Lane 2: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720035) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation