p40-phox Recombinant Rabbit Monoclonal Antibody [JE58-57]
Catalog# HA720040
p40-phox Recombinant Rabbit Monoclonal Antibody [JE58-57]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
p40-phox Recombinant Rabbit Monoclonal Antibody [JE58-57]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human p40-phox aa 290-339/339.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, rat lung tissue lysate, Daudi cell lysate, THP-1 cell lysate, human colon tissue, human spleen tissue, human lung tissue.
Conjugation
unconjugated
Clone Number
JE58-57
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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IHC-P
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1:1,000
Target
Function
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Background References
1. Zhang TP. et. al. Association of NCF2, NCF4, and CYBA Gene Polymorphisms with Rheumatoid Arthritis in a Chinese Population. J Immunol Res. 2020 Oct
2. Ju Z. et. al. The effect of the SNP g.18475 A>G in the 3\'UTR of NCF4 on mastitis susceptibility in dairy cattle. Cell Stress Chaperones. 2018 May
Subcellular Location
Cytoplasm, cytosol, Endosome membrane, Membrane.
Synonyms
CGD3 antibody
MGC3810 antibody
NCF 4 antibody
NCF antibody
NCF-4 antibody
Ncf4 antibody
NCF4_HUMAN antibody
Neutrophil cytosol factor 4 antibody
Neutrophil cytosolic factor 4 antibody
Neutrophil NADPH oxidase factor 4 antibody
ExpandImages
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Western blot analysis of p40-phox on different lysates with Rabbit anti-p40-phox antibody (HA720040) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
Lane 3: Rat spleen tissue lysate (40 µg/Lane)
Lane 4: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720040) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p40-phox antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720040, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-p40-phox antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720040, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-p40-phox antibody (HA720040) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720040) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"