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Glucosidase 2 subunit beta Recombinant Rabbit Monoclonal Antibody [JE58-41]

Usd: 385

Specification

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Catalog# HA720042

Glucosidase 2 subunit beta Recombinant Rabbit Monoclonal Antibody [JE58-41]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Glucosidase 2 subunit beta Recombinant Rabbit Monoclonal Antibody [JE58-41]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Glucosidase 2 subunit beta aa 479-528/528.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 59 kDa

Positive Control

MCF7 cell lysate, HeLa cell lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

JE58-41

RRID

AB_3071982

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

Target

Function

Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia. This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism. View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.

Background References

1. Huang R. et. al. PRKCSH Alternative Splicing Involves in Silica-Induced Expression of Epithelial-Mesenchymal Transition Markers and Cell Proliferation. Dose Response. 2020 May

2. Shin GC. et. al. PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway. Nat Commun. 2019 Jul

Subcellular Location

Endoplasmic reticulum.

UNIPROT

SwissProt: P14314 Human

Synonyms

80K-H protein antibody

AGE-binding receptor 2 antibody

AGE-R2 antibody

G19P1 antibody

GLU2B_HUMAN antibody

Glucosidase 2 subunit beta antibody

Glucosidase II beta subunit antibody

Glucosidase II subunit beta antibody

Hepatocystin antibody

PCLD antibody

Expand

Images

  • Western blot analysis of Glucosidase 2 subunit beta on different lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (<a href="/products/HA720042" style="font-weight: bold;text-decoration: underline;">HA720042</a>) at 1/2,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720042" style="font-weight: bold;text-decoration: underline;">HA720042</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Glucosidase 2 subunit beta on different lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (HA720042) at 1/2,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 80 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720042) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Glucosidase 2 subunit beta on different lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (<a href="/products/HA720042" style="font-weight: bold;text-decoration: underline;">HA720042</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 9 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720042" style="font-weight: bold;text-decoration: underline;">HA720042</a>) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Glucosidase 2 subunit beta on different lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (HA720042) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 80 kDa

    Exposure time: 9 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720042) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Glucosidase 2 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720042" style="font-weight: bold;text-decoration: underline;">HA720042</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Glucosidase 2 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720042, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"