CD79b Recombinant Rabbit Monoclonal Antibody [JE58-34]
Catalog# HA720043
CD79b Recombinant Rabbit Monoclonal Antibody [JE58-34]
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD79b Recombinant Rabbit Monoclonal Antibody [JE58-34]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CD79b aa 1-100/229.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 26 kDa
Positive Control
Daudi cell lysate, Raji cell lysate, human lymph nodes tissue, Daudi.
Conjugation
unconjugated
Clone Number
JE58-34
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
-
1ug/mL
Target
Function
CD79b molecule, immunoglobulin-associated beta, also known as CD79B (Cluster of Differentiation 79B), is a human gene. It is associated with agammaglobulinemia-6. The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.
Background References
1. Ormhøj M. et. al. Chimeric Antigen Receptor T Cells Targeting CD79b Show Efficacy in Lymphoma with or without Cotargeting CD19. Clin Cancer Res. 2019 Dec
2. Takeuchi T. et. al. MYD88, CD79B, and CARD11 gene mutations in CD5-positive diffuse large B-cell lymphoma. Cancer. 2017 Apr
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
AGM6 antibody
B cell antigen receptor complex associated protein beta chain antibody
B cell specific glycoprotein B29 antibody
B-cell antigen receptor complex-associated protein beta chain antibody
B-cell-specific glycoprotein B29 antibody
B29 antibody
B29/Ig-beta/CD79b antibody
CD 79b antibody
CD79b antibody
CD79b antigen (immunoglobulin associated beta) antibody
ExpandImages
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Western blot analysis of CD79b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720043, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue using anti-CD79b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720043, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Daudi cells labeling CD79b.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA720043, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"