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Elp3 Recombinant Rabbit Monoclonal Antibody [JE60-10]

Usd: 385

Specification

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Catalog# HA720046

Elp3 Recombinant Rabbit Monoclonal Antibody [JE60-10]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Elp3 Recombinant Rabbit Monoclonal Antibody [JE60-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Elp3 aa 1-547/547.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

62 kDa

Positive Control

Hela cell lysate, K562 cell lysate, mouse small intestine tissue lysate, rat colon tissue lysate, mouse colon tissue.

Conjugation

unconjugated

Clone Number

JE60-10

RRID

AB_3071986

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P
human
Tested Tested
 
mouse
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).

Background References

1. Li MT. et. al. ELP3 Acetyltransferase is phosphorylated and regulated by the oncogenic anaplastic lymphoma kinase (ALK). Biochem J. 2019 Aug

2. Yang X. et. al. Elongator Protein 3 (Elp3) stabilizes Snail1 and regulates neural crest migration in Xenopus. Sci Rep. 2016 May

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9H9T3 Human

SwissProt: Q9CZX0 Mouse

Entrez Gene: 100362548 Rat

Synonyms

DKFZp761L098 antibody

Elongation protein 3 homolog (S. cerevisiae) antibody

Elongation protein 3 homolog antibody

ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF antibody

elongator acetyltransferase complex subunit 3 antibody

Elongator complex protein 3 antibody

elp3 antibody

ELP3_HUMAN antibody

FLJ10422 antibody

hELP3 antibody

Expand

Images

  • Western blot analysis of Elp3 on different lysates with Rabbit anti-Elp3 antibody (<a href="/products/HA720046" style="font-weight: bold;text-decoration: underline;">HA720046</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (10 µg/lane)<br />Lane 2: K-562 cell lysate (10 µg/lane) <br />Lane 3: mouse small intestine tissue lysate (15 µg/lane)<br />Lane 4: rat colon tissue lysate (15 µg/lane)<br /><br />Exposure time: 2 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA720046" style="font-weight: bold;text-decoration: underline;">HA720046</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa

    Western blot analysis of Elp3 on different lysates with Rabbit anti-Elp3 antibody (HA720046) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (10 µg/lane)
    Lane 2: K-562 cell lysate (10 µg/lane)
    Lane 3: mouse small intestine tissue lysate (15 µg/lane)
    Lane 4: rat colon tissue lysate (15 µg/lane)

    Exposure time: 2 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA720046, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Elp3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720046" style="font-weight: bold;text-decoration: underline;">HA720046</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Elp3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720046, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"