15-PGDH Recombinant Rabbit Monoclonal Antibody [JE60-23]
Catalog# HA720047
15-PGDH Recombinant Rabbit Monoclonal Antibody [JE60-23]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
15-PGDH Recombinant Rabbit Monoclonal Antibody [JE60-23]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human 15-PGDH aa 1-200/266.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 29 kDa
Positive Control
LoVo cell lysates, human placenta tissue lysates, human stomach tissue, mouse bladder tissue.
Conjugation
unconjugated
Clone Number
JE60-23
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. Primary enzyme catalyzing the conversion of hydroxylated arachidonic acid species to their corresponding oxidized metabolites (Probable). Prostaglandin inactivation, catalyzes the first step in the catabolic pathway of the prostaglandins. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Has hydroxylated docosahexaenoic acid metabolites as substrates. Converts resolvins E1, D1 and D2 to their oxo products which represents a mode of resolvins inactivation and stabilizes their anti-inflammatory actions.
Background References
1. Palla AR. et. al. Inhibition of prostaglandin-degrading enzyme 15-PGDH rejuvenates aged muscle mass and strength. Science. 2021 Jan
2. Li Y. et. al. 15-PGDH Expression in Gastric Cancer: A Potential Role in Anti-Tumor Immunity. Cancer Manag Res. 2020 Aug
Subcellular Location
Cytoplasm.
Synonyms
15 hydroxyprostaglandin dehydrogenase [NAD+] antibody
15 PGDH antibody
15-hydroxyprostaglandin dehydrogenase [NAD+] antibody
15-PGDH antibody
15PGDH antibody
Hpgd antibody
Hydroxyprostaglandin dehydrogenase 15 (NAD) antibody
NAD+ dependent 15 hydroxyprostaglandin dehydrogenase antibody
OTTHUMP00000218960 antibody
OTTHUMP00000219016 antibody
ExpandImages
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Western blot analysis of 15-PGDH on LoVo cell lysates with Rabbit anti-15-PGDH antibody (HA720047) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720047) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of 15-PGDH on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720047, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-15-PGDH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720047, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-15-PGDH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720047, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"