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RASA1 Recombinant Rabbit Monoclonal Antibody [JE60-89]

Usd: 385

Specification

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Catalog# HA720052

RASA1 Recombinant Rabbit Monoclonal Antibody [JE60-89]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RASA1 Recombinant Rabbit Monoclonal Antibody [JE60-89]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human RASA1 250-300/1,047.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 116 kDa

Positive Control

HEK-293 cell lysate, A431 cell lysate, A549 cell lysate, HL-60 cell lysate, U-87 MG cell lysate, HeLa cell lysate, Y79 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse placenta tissue lysate, Rat testis tissue lysate, Rat placenta tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

JE60-89

RRID

AB_3071992

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:200

Target

Function

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Background References

1. Revencu N. et. al. RASA1 mosaic mutations in patients with capillary malformation-arteriovenous malformation. J Med Genet. 2020 Jan

2. Gordo G. et. al. Constitutional mosaicism in RASA1-related capillary malformation-arteriovenous malformation. Clin Genet. 2019 Apr

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P20936 Human

SwissProt: P50904 Rat

Entrez Gene: 218397 Mouse

Synonyms

CM AVM antibody

CMAVM antibody

DKFZp434N071 antibody

GAP antibody

GTPase activating protein antibody

GTPase-activating protein antibody

OTTHUMP00000222390 antibody

OTTHUMP00000222391 antibody

OTTHUMP00000222392 antibody

OTTHUMP00000222393 antibody

Expand

Images

  • Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: A431 cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: HL-60 cell lysate (20 µg/Lane)<br />Lane 5: U-87 MG cell lysate (20 µg/Lane)<br />Lane 6: HeLa cell lysate (20 µg/Lane)<br />Lane 7: Y79 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 116 kDa<br />Observed band size: 116 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: A431 cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: HL-60 cell lysate (20 µg/Lane)
    Lane 5: U-87 MG cell lysate (20 µg/Lane)
    Lane 6: HeLa cell lysate (20 µg/Lane)
    Lane 7: Y79 cell lysate (20 µg/Lane)

    Predicted band size: 116 kDa
    Observed band size: 116 kDa

    Exposure time: 1 minute; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/1,000 dilution.<br /><br />Lane 1: Neuro-2a cell lysate<br />Lane 2: PC-12 cell lysate<br />Lane 3: Mouse testis tissue lysate<br />Lane 4: Mouse placenta tissue lysate<br />Lane 5: Rat testis tissue lysate<br />Lane 6: Rat placenta tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 116 kDa<br />Observed band size: 116 kDa<br /><br />Exposure time: 2 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.

    Lane 1: Neuro-2a cell lysate
    Lane 2: PC-12 cell lysate
    Lane 3: Mouse testis tissue lysate
    Lane 4: Mouse placenta tissue lysate
    Lane 5: Rat testis tissue lysate
    Lane 6: Rat placenta tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 116 kDa
    Observed band size: 116 kDa

    Exposure time: 2 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RASA1 antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RASA1 antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RASA1 antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue (low expression) with Rabbit anti-RASA1 antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720052" style="font-weight: bold;text-decoration: underline;">HA720052</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue (low expression) with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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