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MC-2 Recombinant Rabbit Monoclonal Antibody [JE61-09]

Usd: 385

Specification

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Catalog# HA720055

MC-2 Recombinant Rabbit Monoclonal Antibody [JE61-09]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MC-2 Recombinant Rabbit Monoclonal Antibody [JE61-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human MC-2 aa 1-50 / 325.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 37 kDa

Positive Control

SiHa cell lysate, MG-63 cell lysate, U-87 MG cell lysate, mouse hippocampus tissue lysate, rat hippocampus tissue lysate, mouse testis tissue.

Conjugation

unconjugated

Clone Number

JE61-09

RRID

AB_3071995

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins. This gene encodes a member of the seven-pass transmembrane G protein-coupled melanocortin receptor protein family that stimulate cAMP signal transduction. The encoded protein is a receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone and is suggested to play a role in sebum generation.

Background References

1. Trotta MC. et. al. The Melanocortin MC5R as a New Target for Treatment of High Glucose-Induced Hypertrophy of the Cardiac H9c2 Cells. Front Physiol. 2018 Oct

2. Springer MS. et. al. Evolution of the MC5R gene in placental mammals with evidence for its inactivation in multiple lineages that lack sebaceous glands. Mol Phylogenet Evol. 2018 Mar

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P33032 Human

SwissProt: P41149 Mouse

SwissProt: P35345 Rat

Synonyms

MC-2 antibody

MC2 antibody

MC5 R antibody

MC5 Receptor antibody

MC5-R antibody

MC5R antibody

MC5R_HUMAN antibody

Melanocortin 5 receptor antibody

Melanocortin receptor 5 antibody

Images

  • Western blot analysis of MC-2 on different lysates with Rabbit anti-MC-2 antibody (<a href="/products/HA720055" style="font-weight: bold;text-decoration: underline;">HA720055</a>) at 1/2,000 dilution.<br /><br />Lane 1: SiHa cell lysate<br />Lane 2: MG-63 cell lysate<br />Lane 3: U-87 MG cell lysate<br />Lane 4: Mouse hippocampus tissue lysate<br />Lane 5: Rat hippocampus tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720055" style="font-weight: bold;text-decoration: underline;">HA720055</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MC-2 on different lysates with Rabbit anti-MC-2 antibody (HA720055) at 1/2,000 dilution.

    Lane 1: SiHa cell lysate
    Lane 2: MG-63 cell lysate
    Lane 3: U-87 MG cell lysate
    Lane 4: Mouse hippocampus tissue lysate
    Lane 5: Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 48 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720055) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MC-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720055" style="font-weight: bold;text-decoration: underline;">HA720055</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MC-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720055, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"