SAMM50 Recombinant Rabbit Monoclonal Antibody [JE61-49]
Catalog# HA720059
SAMM50 Recombinant Rabbit Monoclonal Antibody [JE61-49]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SAMM50 Recombinant Rabbit Monoclonal Antibody [JE61-49]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SAMM50 aa 341-390/469.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
A431 cell lysate, HeLa cell lysate, C2C12 cell lysate, mouse pancreas tissue lysate, rat pancreas tissue lysate, human pancreas tissue, human kidney tissue, human liver carcinoma tissue, rat testis tissue.
Conjugation
unconjugated
Clone Number
JE61-49
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:200-1:1,000
Target
Function
Sam50 (sorting assembly machinery component 50 homolog, S. cerevisiae), also known as SAMM50, OMP85, TOB55, TRG-3, CGI-51 or YNL026W, is a β-barrel multi-pass membrane protein that belongs to the SAM50/omp85 family of proteins. Localizing to the mitochondrion, Sam50 is believed to function in the assembly pathway of mitochondrial outer membrane β-barrel proteins. More specifically, Sam50 functions as the major component of the SAM (sorting and assembly machinery) complex, also known as the TOB (topogenesis of mitochondrial outer membrane β-barrel proteins) complex, and is required for cell viability. Exposed to the intermembrane space (IMS), the N-terminal POTRA (polypeptide transport-associated) domain of Sam50 (a domain that is conserved from bacteria to man) functions like a receptor for β-barrel proteins. The association of the Sam35 subunit of the SAM complex is essential for Sam50 binding to outer membrane substrate proteins.
Background References
1. Chung GE. et. al. Genetic Polymorphisms of PNPLA3 and SAMM50 Are Associated with Nonalcoholic Fatty Liver Disease in a Korean Population. Gut Liver. 2018 May
2. Utsumi T. et. al. Identification and characterization of protein N-myristoylation occurring on four human mitochondrial proteins, SAMM50, TOMM40, MIC19, and MIC25. PLoS One. 2018 Nov
Subcellular Location
Mitochondrion outer membrane, Mitochondrion, Cytoplasm.
Synonyms
CGI 51 antibody
CGI-51 antibody
FLJ35825 antibody
FLJ42905 antibody
FLJ99036 antibody
OMP85 antibody
SAM50 antibody
SAM50_HUMAN antibody
Samm50 antibody
sorting and assembly machinery 50kDa antibody
ExpandImages
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Western blot analysis of SAMM50 on different lysates with Rabbit anti-SAMM50 antibody (HA720059) at 1/2,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: C2C12 cell lysate (20 µg/Lane)
Lane 4: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 5: Rat pancreas tissue lysate (40 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720059) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SAMM50 antibody (HA720059) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720059) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SAMM50 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720059, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SAMM50 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720059, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SAMM50 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720059, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"