WDR68 Recombinant Rabbit Monoclonal Antibody [JE61-64]
Catalog# HA720062
WDR68 Recombinant Rabbit Monoclonal Antibody [JE61-64]
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
WDR68 Recombinant Rabbit Monoclonal Antibody [JE61-64]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human WDR68 aa 199-248/342.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
39 kDa
Positive Control
Hela cell lysate, 293 cell lysate, mouse lung tissue lysate, SH-SY5Y cell lysate, human prostate tissue, mouse brain tissue, rat epididymis tissue.
Conjugation
unconjugated
Clone Number
JE61-64
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
Target
Function
This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. Alternative splicing results in multiple transcript variants. Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. This protein is involved in the pathway protein ubiquitination, which is part of Protein modification. View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Background References
1. Bonano M. et. al. Molecular characterization of wdr68 gene in embryonic development of Xenopus laevis. Gene Expr Patterns. 2018 Dec
2. Yousefelahiyeh M. et. al. DCAF7/WDR68 is required for normal levels of DYRK1A and DYRK1B. PLoS One. 2018 Nov
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
AN11 antibody
Dcaf7 antibody
DCAF7_HUMAN antibody
DDB1- and CUL4-associated factor 7 antibody
HAN11 antibody
Human anthocyanin antibody
Petunia antibody
Seven WD repeat protein of the AN11 family 1 antibody
SWAN 1 antibody
WD repeat domain 68 antibody
ExpandImages
-
Western blot analysis of WDR68 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720062, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: SH-SY5Y cell lysate -
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720062, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WDR68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720062, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"