Spermine synthase Recombinant Rabbit Monoclonal Antibody [JE61-85]
Catalog# HA720065
Spermine synthase Recombinant Rabbit Monoclonal Antibody [JE61-85]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Spermine synthase Recombinant Rabbit Monoclonal Antibody [JE61-85]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Spermine synthase aa 100-150/366.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
41 kDa
Positive Control
Hela cell lysate, mouse testis tissue lysate, rat uterus tissue lysate, human placenta tissue, human prostate tissue, Hela.
Conjugation
unconjugated
Clone Number
JE61-85
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50
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IHC-P
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1:50-1:200
Target
Function
This gene encodes a protein belonging to the spermidine/spermin synthase family and catalyzes the production of spermine from spermidine. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene cause an X-linked intellectual disability called Snyder-Robinson Syndrome (SRS). Multiple transcript variants encoding different isoforms have been found for this gene. Spermine synthase is an enzyme that converts spermidine into spermine. This enzyme catalyses the following chemical reaction. Spermine synthase is a form of polyamine that is in all eukaryotes and plays a role in a variety of biological functions in plants. Its structure consists of two identical monomers of 41 kDa with three domains each, creating a homodimer formed via dimerization. The interactions between one of the three domains, the N-terminals of the monomers, is responsible for dimerization as that is where the active site is located; the central terminal consisting of four β- strands structurally forming a lid for the third domain, the C-terminal domain.
Background References
1. Guo Y. et. al. Spermine synthase and MYC cooperate to maintain colorectal cancer cell survival by repressing Bim expression. Nat Commun. 2020 Jun
2. Timson DJ. Myosin Va and spermine synthase: partners in exosome transport. Biosci Rep. 2019 Apr
Subcellular Location
Cytosol, extracellular exosome.
Synonyms
MRSR antibody
SMS antibody
Snyder Robinson X linked mental retardation syndrome antibody
Spermidine aminopropyltransferase antibody
Spermine synthase antibody
SPMSY antibody
SpS antibody
SPSY_HUMAN antibody
SRS antibody
Images
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Western blot analysis of Spermine synthase on different lysates with Rabbit anti-Spermine synthase antibody (HA720065) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Mouse testis tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA720065, 1/1,000 in primary antibody dilution buffer (K1803), room temperature for 2 hours.
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 41.3 kDa
Observed band size: 41 kDa -
Western blot analysis of Spermine synthase on rat uterus tissue lysate with Rabbit anti-Spermine synthase antibody (HA720065) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA720065, 1/1,000 in primary antibody dilution buffer (K1803), room temperature for 2 hours.
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 41.3 kDa
Observed band size: 41 kDa -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Spermine synthase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720065, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Spermine synthase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720065, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunocytochemistry analysis of Hela cells labeling Spermine synthase with Rabbit anti-Spermine synthase antibody (HA720065) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Spermine synthase antibody (HA720065) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"