NDRG3 Recombinant Rabbit Monoclonal Antibody [JE61-18]
Catalog# HA720068
NDRG3 Recombinant Rabbit Monoclonal Antibody [JE61-18]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NDRG3 Recombinant Rabbit Monoclonal Antibody [JE61-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human NDRG3 aa 1-35/375.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 41 kDa
Positive Control
LNCaP cell lysate, Raji cell lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat brain tissue lysate, rat testis tissue lysate, human kidney tissue, human small intestine tissue, PC-3M.
Conjugation
unconjugated
Clone Number
JE61-18
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IHC-P
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1:50-1:200
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FC
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1:500-1:1,000
Target
Function
The N-myc downstream regulated gene (NDRG) family is comprised of four members, NDRG1 (also designated Drg1, RTP, rit42, Cap43 and Ndr1), NDRG2, NDRG3 and NDRG4, which share 57-65% homology. The NDRG1 gene, which maps to human chromosome 8q24.3, is evolutionarily conserved and is similarly regulated in humans, mice and rats. Like NDRG2 and NDRG3, NDRG1 is ubiquitously expressed, but it is expressed most prominently in placental membranes and prostate, kidney, small intestine and ovary tissue. NDRG1 gene expression is induced by several compounds, including nickel, and produces a protein involved in stress responses, hormone responses, cell growth and differentiation (1,3,5,6). The gene encoding NDRG3 maps to human chromosome 20q11 and is predominantly expressed in testis, prostate and ovary, which suggests it may play a role in spermatogenesis.
Background References
1. Kim MC. et. al. NDRG3 protein expression is associated with aggressive biologic phenotype and unfavorable outcome in patients with invasive breast cancer. Int J Clin Exp Pathol. 2019 Oct
2. Shi J. et. al. High NDRG3 expression facilitates HCC metastasis by promoting nuclear translocation of beta-catenin. BMB Rep. 2019 Jul
Subcellular Location
Cytoplasm, extracellular exosome.
Synonyms
FLJ13556 antibody
N myc downstream regulated gene 3 antibody
N-myc downstream-regulated gene 3 protein antibody
NDRG family member 3 antibody
NDRG3 antibody
NDRG3_HUMAN antibody
Protein Ndr3 antibody
Protein NDRG3 antibody
Images
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☑ Knockdown (KD)
Western blot analysis of NDRG3 on different lysates with Rabbit anti-NDRG3 antibody (HA720068) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-NDRG3 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 46 kDa
Exposure time: 120 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720068) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of NDRG3 on different lysates with Rabbit anti-NDRG3 antibody (HA720068) at 1/5,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: Raji cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Rat brain tissue lysate
Lane 6: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 46 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720068) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720068, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720068, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of NDRG3 was done on PC-3M cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720068, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"