CAP1 Recombinant Rabbit Monoclonal Antibody [JE61-08]
Catalog# HA720069
CAP1 Recombinant Rabbit Monoclonal Antibody [JE61-08]
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WB
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IHC-P
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IF-Cell
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Human
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Rat
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unconjugated
Overview
Product Name
CAP1 Recombinant Rabbit Monoclonal Antibody [JE61-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CAP1 aa 10-59/475.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 52 kDa
Positive Control
PC-12 cell lysates, Hela cell lysate, A431 cell lysate, HepG2 cell lysate, Raji cell lysate, human colon cancer tissue, human kidney tissue, HepG2, PC-12.
Conjugation
unconjugated
Clone Number
JE61-08
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:100
Target
Function
Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity. The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants encoding different isoforms have been identified.
Background References
1. Dron JS. et. al. A tip of the CAP1 to cholesterol metabolism. Eur Heart J. 2020 Jan
2. Jiang Y. et. al. Mechanism of CAP1-mediated apical actin polymerization in pollen tubes. Proc Natl Acad Sci U S A. 2019 Jun
Subcellular Location
Cell membrane.
Synonyms
Adenylyl cyclase associated protein 1 antibody
Adenylyl cyclase-associated protein 1 antibody
CAP 1 antibody
CAP antibody
CAP, adenylate cyclase associated protein 1 (yeast) antibody
CAP1 antibody
CAP1 PEN antibody
CAP1-PEN antibody
CAP1_HUMAN antibody
Images
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Western blot analysis of CAP1 on PC-12 cell lysates with Rabbit anti-CAP1 antibody (HA720069) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 57 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720069) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CAP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720069, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Raji cell lysate -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CAP1 antibody (HA720069) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720069) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720069, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunocytochemistry analysis of HepG2 cells labeling CAP1 with Rabbit anti-CAP1 antibody (HA720069) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CAP1 antibody (HA720069) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling CAP1 with Rabbit anti-CAP1 antibody (HA720069) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CAP1 antibody (HA720069) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"