Skip to content

NK-p44 Recombinant Rabbit Monoclonal Antibody [JE61-04]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA720070

NK-p44 Recombinant Rabbit Monoclonal Antibody [JE61-04]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NK-p44 Recombinant Rabbit Monoclonal Antibody [JE61-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NK-p44 aa 1-100/276.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

31 kDa

Positive Control

K562 cell lysate, HL-60 cell lysate.

Conjugation

unconjugated

Clone Number

JE61-04

RRID

AB_3072009

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Natural killer (NK) cells direct cytotoxicity against tumor or virally infected cells. NK cell-mediated cytotoxicity is stimulated by several activating receptors associated with the signaling adapter DNAX activation 12/killer cell-activating receptor-associated protein (DAP12). NKp44 is a natural cytotoxicity receptor that is is expressed on IL-2-activated human NK cells and may contribute to the increased efficiency of NK cells to mediate tumor cell lysis. NKp44 is composed of one Ig-like extracellular domain, a transmembrane segment, and a cytoplasmic domain. Prolactin up-regulates and cortisol down-regulates the surface expression of NKp44 at the transcriptional level. A cellular ligand for NKp44 (NKp44L) is expressed during HIV-1 infection and is correlated with the progression of CD4+ T cell depletion and an increase of viral load. This implicates NKp44 as a therapeutic agent that may aid in the progress towards a vaccine for HIV-1 infection.

Background References

1. Ikeda A. et. al. Human NKp44(+) Group 3 Innate Lymphoid Cells Associate with Tumor-Associated Tertiary Lymphoid Structures in Colorectal Cancer. Cancer Immunol Res. 2020 Jun

2. Niehrs A. et. al. A subset of HLA-DP molecules serve as ligands for the natural cytotoxicity receptor NKp44. Nat Immunol. 2019 Sep

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: O95944 Human

Entrez Gene: 367216 Rat

Synonyms

Activating NK receptor NKp44 antibody

CD336 antibody

CD336 antigen antibody

dJ149M18.1 antibody

LY95 antibody

Lymphocyte antigen 95 (activating NK receptor

NK p44) antibody

Lymphocyte antigen 95 homolog (activating NK receptor

NK p44) antibody

Lymphocyte antigen 95 homolog antibody

Expand

Images

  • Western blot analysis of NK-p44 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720070" style="font-weight: bold;text-decoration: underline;">HA720070</a>, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: K562 cell lysate<br />Lane 2: HL-60 cell lysate

    Western blot analysis of NK-p44 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720070, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: K562 cell lysate
    Lane 2: HL-60 cell lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"