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Mitofusin 2 Recombinant Rabbit Monoclonal Antibody [JE59-66]

Usd: 385

Specification

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Catalog# HA720073

Mitofusin 2 Recombinant Rabbit Monoclonal Antibody [JE59-66]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Mitofusin 2 Recombinant Rabbit Monoclonal Antibody [JE59-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Mitofusin 2 aa 51-100/757.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 86 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, Mouse brain tissue lysate, HepG2 cell lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

JE59-66

RRID

AB_3072012

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains. Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.

Background References

1. Hu Y. et. al. The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses. Autophagy. 2020 Apr

2. Rocha AG. et. al. MFN2 agonists reverse mitochondrial defects in preclinical models of Charcot-Marie-Tooth disease type 2A. Science. 2018 Apr

Subcellular Location

Mitochondrion outer membrane.

UNIPROT

SwissProt: O95140 Human

SwissProt: Q80U63 Mouse

SwissProt: Q8R500 Rat

Synonyms

CMT2A antibody

CMT2A2 antibody

CPRP 1 antibody

CPRP1 antibody

EC 3.6.5.- antibody

Fzo antibody

HSG antibody

hyperplasia suppressor gene antibody

Hypertension related protein 1 antibody

KIAA0214 antibody

Expand

Images

  • Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody (<a href="/products/HA720073" style="font-weight: bold;text-decoration: underline;">HA720073</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 cell lysate (20 µg/Lane)<br />Lane 3: HCT 116 cell lysate (20 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 86 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720073" style="font-weight: bold;text-decoration: underline;">HA720073</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody (HA720073) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HEK-293 cell lysate (20 µg/Lane)
    Lane 3: HCT 116 cell lysate (20 µg/Lane)
    Lane 4: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 86 kDa
    Observed band size: 75 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody (<a href="/products/HA720073" style="font-weight: bold;text-decoration: underline;">HA720073</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (10 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 3: Mouse liver tissue lysate (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 86 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 1 minute 21 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720073" style="font-weight: bold;text-decoration: underline;">HA720073</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Mitofusin 2 on different lysates with Rabbit anti-Mitofusin 2 antibody (HA720073) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (10 µg/Lane)
    Lane 2: Mouse brain tissue lysate (20 µg/Lane)
    Lane 3: Mouse liver tissue lysate (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (20 µg/Lane)

    Predicted band size: 86 kDa
    Observed band size: 75 kDa

    Exposure time: 1 minute 21 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation