HMGCS2 Recombinant Rabbit Monoclonal Antibody [JE54-76]
Catalog# HA720083
HMGCS2 Recombinant Rabbit Monoclonal Antibody [JE54-76]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
HMGCS2 Recombinant Rabbit Monoclonal Antibody [JE54-76]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human HMGCS2 aa 459-508/508.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 57 kDa
Positive Control
Caco-2 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat liver tissue, human kidney tissue, Caco-2.
Conjugation
unconjugated
Clone Number
JE54-76
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:100
Target
Function
3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) is an enzyme in humans that is encoded by the HMGCS2 gene. The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the second and rate-limiting reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting, by addition of a third acetyl group to acetoacetyl-CoA, producing HMG-CoA. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. Kim JT. et. al. Regulation of Ketogenic Enzyme HMGCS2 by Wnt/β-catenin/PPARγ Pathway in Intestinal Cells. Cells. 2019 Sep
2. Wang YH. et. al. HMGCS2 Mediates Ketone Production and Regulates the Proliferation and Metastasis of Hepatocellular Carcinoma. Cancers (Basel). 2019 Nov
Subcellular Location
Mitochondrion.
Synonyms
3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
HMCS2_HUMAN antibody
HMG CoA synthase antibody
HMG-CoA synthase antibody
HMGCS 2 antibody
HMGCS2 antibody
Hydroxymethylglutaryl CoA synthase antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of HMGCS2 on different lysates with Rabbit anti-HMGCS2 antibody (HA720083) at 1/2,000 dilution.
Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: HCT 116 cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
Lane 4: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720083) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-HMGCS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720083, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGCS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720083, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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☑ Relative expression (RE)
Immunocytochemistry analysis of Caco-2 (positive) and HCT 116 (negative) labeling HMGCS2 with Rabbit anti-HMGCS2 antibody (HA720083) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HMGCS2 antibody (HA720083) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"