ULBP1 Recombinant Rabbit Monoclonal Antibody [JE65-75]
Catalog# HA720092
ULBP1 Recombinant Rabbit Monoclonal Antibody [JE65-75]
-
WB
-
Human
-
unconjugated
Overview
Product Name
ULBP1 Recombinant Rabbit Monoclonal Antibody [JE65-75]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human ULBP1 aa 1-50/244.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 28 kDa
Positive Control
SiHa cell lysates, Jurkat cell lysates.
Conjugation
unconjugated
Clone Number
JE65-75
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
Target
Function
The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene.
Background References
1. Hu B. et. al. Epithelial-mesenchymal transition may be involved in the immune evasion of circulating gastric tumor cells via downregulation of ULBP1. Cancer Med. 2020 Apr
2. Easom NJW. et. al. ULBP1 Is Elevated in Human Hepatocellular Carcinoma and Predicts Outcome. Front Oncol. 2020 Jun
Subcellular Location
Cell membrane, Endoplasmic reticulum, Membrane.
UNIPROT
Synonyms
Alcan beta antibody
ALCAN-beta antibody
N2DL-1 antibody
N2DL1 antibody
N2DL1_HUMAN antibody
NKG2D ligand 1 antibody
NKG2D ligand 1 precursor antibody
NKG2DL1 antibody
RAET1I antibody
Retinoic acid early transcript 1I antibody
ExpandImages
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Western blot analysis of ULBP1 on SiHa cell lysates with Rabbit anti-ULBP1 antibody (HA720092) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 36 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720092) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ULBP1 on Jurkat cell lysates with Rabbit anti-ULBP1 antibody (HA720092) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 38 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720092) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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