CCDC98 Recombinant Rabbit Monoclonal Antibody [JE62-73]
Catalog# HA720094
CCDC98 Recombinant Rabbit Monoclonal Antibody [JE62-73]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CCDC98 Recombinant Rabbit Monoclonal Antibody [JE62-73]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CCDC98 aa 360-409/409.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 47 kDa
Positive Control
K562 cell lysate, Daudi cell lysate, SH-SY5Y, human rectum tissue, human tonsil tissue, SiHa.
Conjugation
unconjugated
Clone Number
JE62-73
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:100-1:500
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FC
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1:50-1:100
Target
Function
This gene encodes a protein that binds to the C-terminal repeats of breast cancer 1 (BRCA1) through a phospho-SXXF motif. The encoded protein recruits ubiquitin interaction motif containing 1 protein to BRCA1 protein and is required for DNA damage resistance, DNA repair, and cell cycle checkpoint control. Pseudogenes of this gene are found on chromosomes 3 and 8. Alternative splicing results in multiple transcript variants. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.
Background References
1. Bose M.et. al. BRCA1 mislocalization leads to aberrant DNA damage response in heterozygous ABRAXAS1 mutation carrier cells. Hum Mol Genet. 2019 Dec
2. Bąk A.et. al. Searching for new breast cancer-associated genes. ABRAXAS1 gene mutations in the group of BRCA1-negative patients. Pol J Pathol. 2018
Subcellular Location
Nucleus.
UNIPROT
Synonyms
3830405G04Rik antibody
5630400M01Rik antibody
ABRA1 antibody
ABRAXAS antibody
Abraxas protein antibody
AI506069 antibody
AL024423 antibody
AV118690 antibody
BRCA1-A complex subunit Abraxas antibody
coiled coil domain containing 98 antibody
ExpandImages
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Western blot analysis of CCDC98 on different lysates with Rabbit anti-CCDC98 antibody (HA720094) at 1/5,000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA720094, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46.7 kDa
Observed band size: 47 kDa -
ICC staining of CCDC98 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720094, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-CCDC98 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720094, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCDC98 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720094, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of CCDC98 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720094, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"