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Sterol carrier protein 2 Recombinant Rabbit Monoclonal Antibody [JE62-79]

Usd: 385

Specification

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Catalog# HA720099

Sterol carrier protein 2 Recombinant Rabbit Monoclonal Antibody [JE62-79]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Sterol carrier protein 2 Recombinant Rabbit Monoclonal Antibody [JE62-79]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Sterol carrier protein 2 aa 14-64/547.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 59 kDa

Positive Control

SK-Br-3 cell lysate, THP-1 cell lysate, HL-60 cell lysate, human small intestine tissue, mouse spleen tissue.

Conjugation

unconjugated

Clone Number

JE62-79

RRID

AB_3072038

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5000

  • IHC-P

  • 1:100-1:500

Target

Function

This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.

Background References

1. Ding X.et. al. SCP2-mediated cholesterol membrane trafficking promotes the growth of pituitary adenomas via Hedgehog signaling activation. J Exp Clin Cancer Res. 2019 Sep

2. Kiema TR.et. al. The peroxisomal zebrafish SCP2-thiolase (type-1) is a weak transient dimer as revealed by crystal structures and native mass spectrometry. Biochem J. 2019 Jan

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Mitochondrion, Peroxisome.

UNIPROT

SwissProt: P22307 Human

SwissProt: P32020 Mouse

Synonyms

DKFZp686C12188 antibody

DKFZp686D11188 antibody

NLTP antibody

NLTP_HUMAN antibody

Non-specific lipid-transfer protein antibody

Nonspecific lipid transfer protein antibody

NSL TP antibody

NSL-TP antibody

OTTHUMP00000010488 antibody

OTTHUMP00000231766 antibody

Expand

Images

  • Western blot analysis of Sterol carrier protein 2 on different lysates with Rabbit anti-Sterol carrier protein 2 antibody (<a href="/products/HA720099" style="font-weight: bold;text-decoration: underline;">HA720099</a>) at 1/5,000 dilution.<br /><br />Lane 1: SK-BR-3 (Human breast cancer cell) cell lysate<br />Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 30 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA720099" style="font-weight: bold;text-decoration: underline;">HA720099</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa

    Western blot analysis of Sterol carrier protein 2 on different lysates with Rabbit anti-Sterol carrier protein 2 antibody (HA720099) at 1/5,000 dilution.

    Lane 1: SK-BR-3 (Human breast cancer cell) cell lysate
    Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA720099, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Sterol carrier protein 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720099" style="font-weight: bold;text-decoration: underline;">HA720099</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Sterol carrier protein 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720099, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Sterol carrier protein 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720099" style="font-weight: bold;text-decoration: underline;">HA720099</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Sterol carrier protein 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720099, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"