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NCAPD2 Recombinant Rabbit Monoclonal Antibody [JE62-22]

Usd: 385

Specification

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Catalog# HA720100

NCAPD2 Recombinant Rabbit Monoclonal Antibody [JE62-22]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NCAPD2 Recombinant Rabbit Monoclonal Antibody [JE62-22]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NCAPD2 aa 51-200/1,401.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 157 kDa

Positive Control

Hela cell lysate, HL-60 cell lysate, SiHa cell lysate, human tonsil tissue, SiHa.

Conjugation

unconjugated

Clone Number

JE62-22

RRID

AB_3072039

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.

Background References

1. Lin Y.et. al. A novel homozygous splice-site variant of NCAPD2 gene identified in two siblings with primary microcephaly: The second case report. Clin Genet. 2019 Jul

Subcellular Location

Nucleus, Cytoplasm, Chromosome.

UNIPROT

SwissProt: Q15021 Human

Synonyms

2810406C15Rik antibody

2810465G24Rik antibody

CAP-D2 antibody

CAPD2 antibody

CEP2 antibody

CEP250 antibody

Chromosome condensation-related SMC-associated protein 1 antibody

Chromosome-associated protein D2 antibody

CNAP1 antibody

CND1 antibody

Expand

Images

  • Western blot analysis of NCAPD2 on different lysates with Rabbit anti-NCAPD2 antibody (<a href="/products/HA720100" style="font-weight: bold;text-decoration: underline;">HA720100</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: SiHa (Human cervical cancersSquamous cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 2 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA720100" style="font-weight: bold;text-decoration: underline;">HA720100</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 157 kDa<br />Observed band size: 157 kDa

    Western blot analysis of NCAPD2 on different lysates with Rabbit anti-NCAPD2 antibody (HA720100) at 1/1,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: SiHa (Human cervical cancersSquamous cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 2 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA720100, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 157 kDa
    Observed band size: 157 kDa

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCAPD2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA720100" style="font-weight: bold;text-decoration: underline;">HA720100</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCAPD2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720100, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining of NCAPD2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA720100" style="font-weight: bold;text-decoration: underline;">HA720100</a>, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).<br /><br />Secondary antibody only control: Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.

    ICC staining of NCAPD2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720100, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"