ALDH16A1 Recombinant Rabbit Monoclonal Antibody [JE62-68]
Catalog# HA720104
ALDH16A1 Recombinant Rabbit Monoclonal Antibody [JE62-68]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ALDH16A1 Recombinant Rabbit Monoclonal Antibody [JE62-68]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human ALDH16A1 aa 550-600/802.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 85 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
JE62-68
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
This gene encodes a member of the aldehyde dehydrogenase superfamily. The family members act on aldehyde substrates and use nicotinamide adenine dinucleotide phosphate (NADP) as a cofactor. This gene is conserved in chimpanzee, dog, cow, mouse, rat, and zebrafish. The protein encoded by this gene interacts with maspardin, a protein that when truncated is responsible for Mast syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. Pantouris G.et. al. Expression, purification and crystallization of the novel Xenopus tropicalis ALDH16B1, a homologue of human ALDH16A1. Chem Biol Interact. 2019 May
2. Charkoftaki G.et. al. Transcriptomic analysis and plasma metabolomics in Aldh16a1-null mice reveals a potential role of ALDH16A1 in renal function. Chem Biol Interact. 2017 Oct
Subcellular Location
Membrane.
Synonyms
Aldehyde dehydrogenase 16 family, member A1 antibody
Aldehyde dehydrogenase family 16 member A1 antibody
ALDH16A1 aldehyde dehydrogenase 16 family, member A1 antibody
Aldh16a1 antibody
MGC10204 antibody
Images
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Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody (HA720104) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
Lane 5: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 85 kDa
Observed band size: 75 kDa
Exposure time: 1 minute 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720104) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody (HA720104) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ALDH16A1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 80 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720104) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"