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iFluor™ 594 Conjugated Cytokeratin 16 Recombinant Rabbit Monoclonal Antibody [SC52-09]

Usd: 429

Specification

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Catalog# HA720114F

iFluor™ 594 Conjugated Cytokeratin 16 Recombinant Rabbit Monoclonal Antibody [SC52-09]

Application

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • iFluor™ 594

Overview

Product Name

iFluor™ 594 Conjugated Cytokeratin 16 Recombinant Rabbit Monoclonal Antibody [SC52-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Cytokeratin 16 aa 27-75.

Species Reactivity

Human

Validated Applications

IF-Cell, IF-Tissue

Target Molecular Weight

Predicted band size: 51 kDa

Positive Control

A431, human esophagus tissue, human skin tissue.

Conjugation

iFluor™ 594

Clone Number

SC52-09

RRID

AB_3072053

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:50

  • IF-Tissue

  • 1:50

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN).

Background References

1. Pal SK. et. al. The expression profiles of acidic epithelial keratins in ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol 115:523-31 (2013).

2. Cheng CH. et. al. Keratin gene expression profiles after digit amputation in C57BL/6 vs. regenerative MRL mice imply an early regenerative keratinocyte activated-like state. Physiol Genomics 45:409-21 (2013).

Subcellular Location

Cytoskeleton, Nucleus.

UNIPROT

SwissProt: P08779 Human

Synonyms

CK 16 antibody

CK-16 antibody

CK16 antibody

Cytokeratin-16 antibody

Cytokeratin16 antibody

FNEPPK antibody

Focal non epidermolytic palmoplantar keratoderma antibody

K 16 antibody

K16 antibody

K1C16_HUMAN antibody

Expand

Images

  • Immunocytochemistry analysis of A431 cells labeling Cytokeratin 16.<br />Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with <a href="/products/HA720114F" style="font-weight: bold;text-decoration: underline;">HA720114F</a> at 1/50 dilution Rabbit monoclonal to Cytokeratin 16 (iFluor&trade; 594)(shown in red). DAPI was used as nuclear counterstain.

    Immunocytochemistry analysis of A431 cells labeling Cytokeratin 16.
    Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720114F at 1/50 dilution Rabbit monoclonal to Cytokeratin 16 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16.<br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with <a href="/products/HA720114F" style="font-weight: bold;text-decoration: underline;">HA720114F</a> Cytokeratin 16 (iFluor&trade; 594, red) at 1/50 dilution, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16.
    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720114F Cytokeratin 16 (iFluor™ 594, red) at 1/50 dilution, washed with PBS. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 16.<br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with <a href="/products/HA720114F" style="font-weight: bold;text-decoration: underline;">HA720114F</a> Cytokeratin 16 (iFluor&trade; 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 16.
    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720114F Cytokeratin 16 (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"