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iFluor™ 594 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]

Usd: 429

Specification

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Catalog# HA720118F

iFluor™ 594 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]

Application

  • IF-Tissue

  • IF-Cell

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

Predicted species support after-sales service
Conjugation

  • iFluor™ 594

Overview

Product Name

iFluor™ 594 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483.

Species Reactivity

Human (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

IF-Tissue, IF-Cell, FC

Molecular Weight

Predicted band size: 54 kDa

Positive Control

Human breast tissue, human liver tissue, SK-Br-3, A431.

Conjugation

iFluor™ 594

Clone Number

SU0338

RRID

AB_3072057

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Tissue

  • 1:100-1:400

  • IF-Cell

  • 1:50

  • FC

  • 1:500-1:1,000

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy.

Background References

1. Ruiz A et al. Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis. Cell Death Dis 7:e2059 (2016).

2. Xiao, L. et al. Three-dimensional epithelial and mesenchymal cell co-cultures form early tooth epithelium invagination-like structures: expression patterns of relevant molecules. J. Cell. Biochem. 113: 1875-1885(2012).

Subcellular Location

Nucleoplasm, Nucleus matrix, Cytoplasm.

UNIPROT

SwissProt: P05787 Human

SwissProt: P11679 Mouse

Synonyms

CARD2 antibody

Cell proliferation inducing gene 46 protein antibody

Cell proliferation inducing protein 46 antibody

CK 8 antibody

CK-8 antibody

CK8 antibody

CYK8 antibody

Cytokeratin 8 antibody

Cytokeratin-8 antibody

K2C8 antibody

Expand

Images

  • Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (<a href="/products/HA720118F" style="font-weight: bold;text-decoration: underline;">HA720118F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (<a href="/products/HA720118F" style="font-weight: bold;text-decoration: underline;">HA720118F</a>, red) at 1/400 dilution at +4℃ overnight, washed with PBS.<br /><br />Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (HA720118F).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720118F, red) at 1/400 dilution at +4℃ overnight, washed with PBS.

    Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (<a href="/products/HA720118F" style="font-weight: bold;text-decoration: underline;">HA720118F</a>) and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (<a href="/products/HA720118F" style="font-weight: bold;text-decoration: underline;">HA720118F</a>, red) at 1/100 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, green) at 1/400 dilution at +4℃ overnight, washed with PBS.<br /><br />Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 8 (HA720118F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720118F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS.

    Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 (<a href="/products/HA720118F" style="font-weight: bold;text-decoration: underline;">HA720118F</a>).<br /><br />Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with Cytokeratin 8 (<a href="/products/HA720118F" style="font-weight: bold;text-decoration: underline;">HA720118F</a>, red) at 1/50 dilution and Beta-tubulin (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>, yellow) at 1/400 dilution. <br /><br />Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 (HA720118F).

    Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with Cytokeratin 8 (HA720118F, red) at 1/50 dilution and Beta-tubulin (EM0103, yellow) at 1/400 dilution.

    Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Flow cytometric analysis of A431 cells labeling Cytokeratin 8.<br /><br />Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 8 (<a href="/products/HA720118F" style="font-weight: bold;text-decoration: underline;">HA720118F</a>, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor&trade; 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling Cytokeratin 8.

    Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 8 (HA720118F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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