iFluor™ 594 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]
Catalog# HA720124F
iFluor™ 594 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]
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IF-Tissue
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Human
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Mouse
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Rat
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iFluor™ 594
Overview
Product Name
iFluor™ 594 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within mouse Cytokeratin 1 aa 586-637.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Tissue
Molecular Weight
66 kDa
Positive Control
Human skin tissue.
Conjugation
iFluor™ 594
Clone Number
SN72-08
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:100
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis.
Background References
1. Zhu, H. et al. 2016. MiR-217 is down-regulated in psoriasis and promotes keratinocyte differentiation via targeting GRHL2. Biochemical and biophysical research communications. 471: 169-76.
2. Li, G. et al. 2015. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. The Journal of biological chemistry. 290: 20448-54.
Subcellular Location
Cell membrane.
Synonyms
67 kDa cytokeratin antibody
CK-1 antibody
CK1 antibody
Cytokeratin-1 antibody
Cytokeratin1 antibody
EHK antibody
EHK1 antibody
Epidermolytic hyperkeratosis 1 antibody
EPPK antibody
Hair alpha protein antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720124F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720124F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"